Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Sus scrofa | |
NADH | stimulates H2O2 production reaction of the enzyme | Cavia porcellus | |
NADH | stimulates H2O2 production reaction of the enzyme | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | - |
Cavia porcellus | |
H2O2 | - |
Sus scrofa | |
NAD+ | inhibits H2O2 production reaction of the enzyme | Cavia porcellus | |
NAD+ | inhibits H2O2 production reaction of the enzyme | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Cavia porcellus | 5739 | - |
mitochondrion | - |
Sus scrofa | 5739 | - |
synaptosome | - |
Cavia porcellus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates both catalytic function and H2O2 production | Cavia porcellus | |
Ca2+ | activates both catalytic function and H2O2 production | Sus scrofa | |
Mg2+ | - |
Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | Cavia porcellus | - |
[dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
ir | |
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | Sus scrofa | - |
[dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
ir | |
additional information | Sus scrofa | enzyme also catalyzes the production of H2O2 from 2-oxoglutarate with CoA in absence of NAD+, which inhibits this reaction, enzyme is a target for reactive oxygen species and also contributes to generation of oxidative stress in mitochondria when NADH oxidation is impaired, overview | ? | - |
? | |
additional information | Cavia porcellus | enzyme also catalyzes the production of H2O2 in vivo, in vitro, and in situ from 2-oxoglutarate with CoA in absence of NAD+, which inhibits this reaction, enzyme is a atrget for reactive oxygen species and also contributes to generation of oxidative stress in mitochondria when NADH oxidation is impaired, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme is further purified from commercial pig heart preparation | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain cortex | - |
Cavia porcellus | - |
commercial preparation | from heart | Sus scrofa | - |
heart | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sus scrofa |
additional information | - |
activity under different conditions in synaptosomes | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CoA | - |
Cavia porcellus | ? + H2O2 | - |
? | |
2-oxoglutarate + CoA | - |
Sus scrofa | ? + H2O2 | - |
? | |
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | - |
Cavia porcellus | [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
ir | |
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | - |
Sus scrofa | [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
ir | |
additional information | enzyme also catalyzes the production of H2O2 from 2-oxoglutarate with CoA in absence of NAD+, which inhibits this reaction, enzyme is a target for reactive oxygen species and also contributes to generation of oxidative stress in mitochondria when NADH oxidation is impaired, overview | Sus scrofa | ? | - |
? | |
additional information | enzyme also catalyzes the production of H2O2 in vivo, in vitro, and in situ from 2-oxoglutarate with CoA in absence of NAD+, which inhibits this reaction, enzyme is a atrget for reactive oxygen species and also contributes to generation of oxidative stress in mitochondria when NADH oxidation is impaired, overview | Cavia porcellus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-ketoglutarate dehydrogenase | - |
Cavia porcellus |
alpha-ketoglutarate dehydrogenase | - |
Sus scrofa |
alpha-KGDH | - |
Cavia porcellus |
alpha-KGDH | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
37 | - |
assay at, synaptosomes | Cavia porcellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Sus scrofa |
7.4 | - |
assay at, synaptosomes | Cavia porcellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cavia porcellus | |
NAD+ | - |
Sus scrofa | |
thiamine diphosphate | - |
Cavia porcellus | |
thiamine diphosphate | - |
Sus scrofa |