BRENDA - Enzyme Database
show all sequences of 1.2.1.97

Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1

Felux A.-K, F.A.; Spiteller D, S.D.; Klebensberger J, K.J.; Schleheck D, S.D.; Proc. Natl. Acad. Sci. USA 112, E4298-E4305 (2015)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
(2S)-3-sulfolactaldehyde
with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication; with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(2S)-3-sulfolactaldehyde + NAD+ + H2O
Pseudomonas putida
-
(2S)-3-sulfolactate + NADH + H+
-
-
?
(2S)-3-sulfolactaldehyde + NAD+ + H2O
Pseudomonas putida SQ1
-
(2S)-3-sulfolactate + NADH + H+
-
-
?
(2S)-3-sulfolactaldehyde + NADP+ + H2O
Pseudomonas putida
-
(2S)-3-sulfolactate + NADPH + H+
-
-
?
(2S)-3-sulfolactaldehyde + NADP+ + H2O
Pseudomonas putida SQ1
-
(2S)-3-sulfolactate + NADPH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida SQ1
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2S)-3-sulfolactaldehyde + NAD+ + H2O
-
735194
Pseudomonas putida
(2S)-3-sulfolactate + NADH + H+
-
-
-
?
(2S)-3-sulfolactaldehyde + NAD+ + H2O
-
735194
Pseudomonas putida SQ1
(2S)-3-sulfolactate + NADH + H+
-
-
-
?
(2S)-3-sulfolactaldehyde + NADP+ + H2O
-
735194
Pseudomonas putida
(2S)-3-sulfolactate + NADPH + H+
-
-
-
?
(2S)-3-sulfolactaldehyde + NADP+ + H2O
-
735194
Pseudomonas putida SQ1
(2S)-3-sulfolactate + NADPH + H+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
SLA dehydrogenase
-
Pseudomonas putida
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11.4
-
(2S)-3-sulfolactaldehyde
with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication
Pseudomonas putida
11.8
-
(2S)-3-sulfolactaldehyde
with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
NADP+
-
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
NADP+
-
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
(2S)-3-sulfolactaldehyde
with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication; with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(2S)-3-sulfolactaldehyde + NAD+ + H2O
Pseudomonas putida
-
(2S)-3-sulfolactate + NADH + H+
-
-
?
(2S)-3-sulfolactaldehyde + NAD+ + H2O
Pseudomonas putida SQ1
-
(2S)-3-sulfolactate + NADH + H+
-
-
?
(2S)-3-sulfolactaldehyde + NADP+ + H2O
Pseudomonas putida
-
(2S)-3-sulfolactate + NADPH + H+
-
-
?
(2S)-3-sulfolactaldehyde + NADP+ + H2O
Pseudomonas putida SQ1
-
(2S)-3-sulfolactate + NADPH + H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2S)-3-sulfolactaldehyde + NAD+ + H2O
-
735194
Pseudomonas putida
(2S)-3-sulfolactate + NADH + H+
-
-
-
?
(2S)-3-sulfolactaldehyde + NAD+ + H2O
-
735194
Pseudomonas putida SQ1
(2S)-3-sulfolactate + NADH + H+
-
-
-
?
(2S)-3-sulfolactaldehyde + NADP+ + H2O
-
735194
Pseudomonas putida
(2S)-3-sulfolactate + NADPH + H+
-
-
-
?
(2S)-3-sulfolactaldehyde + NADP+ + H2O
-
735194
Pseudomonas putida SQ1
(2S)-3-sulfolactate + NADPH + H+
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11.4
-
(2S)-3-sulfolactaldehyde
with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication
Pseudomonas putida
11.8
-
(2S)-3-sulfolactaldehyde
with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication
Pseudomonas putida
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
81.4
-
(2S)-3-sulfolactaldehyde
with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication
Pseudomonas putida
84.2
-
(2S)-3-sulfolactaldehyde
with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication
Pseudomonas putida
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
81.4
-
(2S)-3-sulfolactaldehyde
with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication
Pseudomonas putida
84.2
-
(2S)-3-sulfolactaldehyde
with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication
Pseudomonas putida
Other publictions for EC 1.2.1.97
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735194
Felux A.-K
Entner-Doudoroff pathway for s ...
Pseudomonas putida, Pseudomonas putida SQ1
Proc. Natl. Acad. Sci. USA
112
E4298-E4305
2015
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