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show all sequences of 1.2.1.69

Isolation of an aldehyde dehydrogenase involved in the oxidation of fluoroacetaldehyde to fluoroacetate in Streptomyces cattleya

Murphy, C.D.; Moss, S.J.; O'Hagan, D.; Appl. Environ. Microbiol. 67, 4919-4921 (2001)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
1 mM, increase in activity
Streptomyces cattleya
Inhibitors
Inhibitors
Commentary
Organism
Structure
Chloroacetaldehyde
0.25 mM, time-dependent inactivation
Streptomyces cattleya
Cu2+
1 mM, strong inhibition
Streptomyces cattleya
iodoacetamide
0.05 mM, strong inhibition
Streptomyces cattleya
Mg2+
1 mM, 80% loss of activity, possibly due to a decreased dissociation rate of NADH from the enzyme
Streptomyces cattleya
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
fluoroacetaldehyde
-
Streptomyces cattleya
0.15
-
glycoaldehyde
-
Streptomyces cattleya
0.32
-
benzaldehyde
-
Streptomyces cattleya
0.81
-
acetaldehyde
-
Streptomyces cattleya
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
4 * 55000, SDS-PAGE
Streptomyces cattleya
200000
-
gel filtration
Streptomyces cattleya
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
fluroracetaldehyde + NAD+ + H2O
Streptomyces cattleya
involved in fluorometabolite biosynthesis
fluoroacetate + NADH
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces cattleya
-
NRRL 8057
-
Streptomyces cattleya NRRL 8057
-
NRRL 8057
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, anion-exchange, 5'-AMP-agarose
Streptomyces cattleya
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.889
-
-
Streptomyces cattleya
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NAD+ + H2O
40% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya
acetate + NADH + H+
-
636460
Streptomyces cattleya
?
acetaldehyde + NAD+ + H2O
40% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya NRRL 8057
acetate + NADH + H+
-
636460
Streptomyces cattleya NRRL 8057
?
benzaldehyde + NAD+ + H2O
65% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya
benzoate + NADH + H+
-
636460
Streptomyces cattleya
?
benzaldehyde + NAD+ + H2O
65% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya NRRL 8057
benzoate + NADH + H+
-
636460
Streptomyces cattleya NRRL 8057
?
chloroacetaldehyde + NAD+ + H2O
80% of activity with fluoroacetaldehyde, inactivation upon extended incubation
636460
Streptomyces cattleya
chloroacetate + NADH + H+
-
636460
Streptomyces cattleya
?
chloroacetaldehyde + NAD+ + H2O
80% of activity with fluoroacetaldehyde, inactivation upon extended incubation
636460
Streptomyces cattleya NRRL 8057
chloroacetate + NADH + H+
-
636460
Streptomyces cattleya NRRL 8057
?
DL-glyceraldehyde + NAD+ + H2O
10% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya
DL-glycerate + NADH + H+
-
636460
Streptomyces cattleya
?
fluroracetaldehyde + NAD+ + H2O
-
636460
Streptomyces cattleya
fluoroacetate + NADH + H+
-
636460
Streptomyces cattleya
?
fluroracetaldehyde + NAD+ + H2O
-
636460
Streptomyces cattleya NRRL 8057
fluoroacetate + NADH + H+
-
636460
Streptomyces cattleya NRRL 8057
?
fluroracetaldehyde + NAD+ + H2O
involved in fluorometabolite biosynthesis
636460
Streptomyces cattleya
fluoroacetate + NADH
-
-
-
-
glycoaldehyde + NAD+ + H2O
150% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya
glycolate + NADH + H+
-
636460
Streptomyces cattleya
?
propionaldehyde + NAD+ + H2O
28% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya
propionate + NADH + H+
-
636460
Streptomyces cattleya
?
propionaldehyde + NAD+ + H2O
28% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya NRRL 8057
propionate + NADH + H+
-
636460
Streptomyces cattleya NRRL 8057
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 55000, SDS-PAGE
Streptomyces cattleya
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Streptomyces cattleya
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
1 mM, increase in activity
Streptomyces cattleya
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Chloroacetaldehyde
0.25 mM, time-dependent inactivation
Streptomyces cattleya
Cu2+
1 mM, strong inhibition
Streptomyces cattleya
iodoacetamide
0.05 mM, strong inhibition
Streptomyces cattleya
Mg2+
1 mM, 80% loss of activity, possibly due to a decreased dissociation rate of NADH from the enzyme
Streptomyces cattleya
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
fluoroacetaldehyde
-
Streptomyces cattleya
0.15
-
glycoaldehyde
-
Streptomyces cattleya
0.32
-
benzaldehyde
-
Streptomyces cattleya
0.81
-
acetaldehyde
-
Streptomyces cattleya
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
4 * 55000, SDS-PAGE
Streptomyces cattleya
200000
-
gel filtration
Streptomyces cattleya
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
fluroracetaldehyde + NAD+ + H2O
Streptomyces cattleya
involved in fluorometabolite biosynthesis
fluoroacetate + NADH
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, anion-exchange, 5'-AMP-agarose
Streptomyces cattleya
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.889
-
-
Streptomyces cattleya
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NAD+ + H2O
40% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya
acetate + NADH + H+
-
636460
Streptomyces cattleya
?
acetaldehyde + NAD+ + H2O
40% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya NRRL 8057
acetate + NADH + H+
-
636460
Streptomyces cattleya NRRL 8057
?
benzaldehyde + NAD+ + H2O
65% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya
benzoate + NADH + H+
-
636460
Streptomyces cattleya
?
benzaldehyde + NAD+ + H2O
65% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya NRRL 8057
benzoate + NADH + H+
-
636460
Streptomyces cattleya NRRL 8057
?
chloroacetaldehyde + NAD+ + H2O
80% of activity with fluoroacetaldehyde, inactivation upon extended incubation
636460
Streptomyces cattleya
chloroacetate + NADH + H+
-
636460
Streptomyces cattleya
?
chloroacetaldehyde + NAD+ + H2O
80% of activity with fluoroacetaldehyde, inactivation upon extended incubation
636460
Streptomyces cattleya NRRL 8057
chloroacetate + NADH + H+
-
636460
Streptomyces cattleya NRRL 8057
?
DL-glyceraldehyde + NAD+ + H2O
10% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya
DL-glycerate + NADH + H+
-
636460
Streptomyces cattleya
?
fluroracetaldehyde + NAD+ + H2O
-
636460
Streptomyces cattleya
fluoroacetate + NADH + H+
-
636460
Streptomyces cattleya
?
fluroracetaldehyde + NAD+ + H2O
-
636460
Streptomyces cattleya NRRL 8057
fluoroacetate + NADH + H+
-
636460
Streptomyces cattleya NRRL 8057
?
fluroracetaldehyde + NAD+ + H2O
involved in fluorometabolite biosynthesis
636460
Streptomyces cattleya
fluoroacetate + NADH
-
-
-
-
glycoaldehyde + NAD+ + H2O
150% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya
glycolate + NADH + H+
-
636460
Streptomyces cattleya
?
propionaldehyde + NAD+ + H2O
28% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya
propionate + NADH + H+
-
636460
Streptomyces cattleya
?
propionaldehyde + NAD+ + H2O
28% of activity with fluoroacetaldehyde
636460
Streptomyces cattleya NRRL 8057
propionate + NADH + H+
-
636460
Streptomyces cattleya NRRL 8057
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 55000, SDS-PAGE
Streptomyces cattleya
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Streptomyces cattleya
Other publictions for EC 1.2.1.69
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
636461
Murphy
Fluorinated natural products: ...
Streptomyces cattleya
Chemosphere
52
455-461
2003
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2
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1
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4
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636460
Murphy
Isolation of an aldehyde dehyd ...
Streptomyces cattleya, Streptomyces cattleya NRRL 8057
Appl. Environ. Microbiol.
67
4919-4921
2001
1
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5
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1
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13
1
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1
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1
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4
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13
1
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