BRENDA - Enzyme Database
show all sequences of 1.2.1.65

Purification and characterization of NAD+-dependent salicylaldehyde dehydrogenase from carbaryl-degrading Pseudomonas sp. strain C6

Singh, R.; Trivedi, V.D.; Phale, P.S.; Appl. Biochem. Biotechnol. 172, 806-819 (2014)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
40% inhibition at 1 mM
Pseudomonas sp.
2,2'-dipyridyl
50% inhibition at 1 mM
Pseudomonas sp.
2-naphthaldehyde
substrate inhibition
Pseudomonas sp.
3-Chlorobenzaldehyde
substrate inhibition
Pseudomonas sp.
3-Hydroxybenzaldehyde
substrate inhibition
Pseudomonas sp.
4-Chlorobenzaldehyde
substrate inhibition
Pseudomonas sp.
4-nitrobenzaldehyde
substrate inhibition
Pseudomonas sp.
benzaldehyde
substrate inhibition
Pseudomonas sp.
EDTA
50% inhibition at 50 mM
Pseudomonas sp.
EGTA
60% inhibition at 50 mM
Pseudomonas sp.
Na+
inhibitory at higher concentration
Pseudomonas sp.
Salicylaldehyde
substrate inhibition, 86% and 70% decrease in activity at 0.05 mM and 0.1 mM, respectively
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics for NAD+
Pseudomonas sp.
0.001
-
benzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.0021
-
3-Hydroxybenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.0035
-
2-naphthaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.0039
-
3-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.0045
-
Salicylaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.0064
-
4-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.011
-
4-nitrobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.052
-
NAD+
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
the enzyme contains 1.8 mol/mol of enzyme
Pseudomonas sp.
Fe2+
the enzyme contains 0.4 mol/mol of enzyme
Pseudomonas sp.
K+
44% activating at 200 mM
Pseudomonas sp.
Mg2+
the enzyme contains 0.3 mol/mol of enzyme
Pseudomonas sp.
additional information
external addition of Ca2+, Mg2+, Fe2+ and Fe3+ at 10 mM, either as sulfate or chloride salts, fail to show an increase in the enzyme activity
Pseudomonas sp.
Zn2+
the enzyme contains 0.2 mol/mol of enzyme
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
150000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
salicylaldehyde + NAD+ + H2O
Pseudomonas sp.
-
salicylate + NADH + 2 H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Purification (Commentary)
Commentary
Organism
native enzyme 28fold from cellfree extract by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography, and gel filtration
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
36.3
-
purified native enzyme, pH 8.0, temperature not specified in the publication
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-naphthaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
1-naphthoate + NADH + 2 H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
2-carboxybenzoate + NADH + 2 H+
-
-
-
?
2-chlorobenzaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
2-chlorobenzoate + NADH + 2 H+
-
-
-
?
2-methoxybenzaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
2-methoxybenzoate + NADH + 2 H+
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
moderate activity
741638
Pseudomonas sp.
2-naphthoate + NADH + 2 H+
-
-
-
?
2-nitrobenzaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
2-nitrobenzoate + NADH + 2 H+
-
-
-
?
3-carboxybenzaldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
3-carboxybenzoate + NADH + 2 H+
-
-
-
?
3-chlorobenzaldehyde + NAD+ + H2O
moderate activity
741638
Pseudomonas sp.
3-chlorobenzoate + NADH + 2 H+
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
low to moderate activity
741638
Pseudomonas sp.
3-hydroxybenzoate + NADH + 2 H+
-
-
-
?
3-methoxybenzaldehyde + NAD+ + H2O
moderate activity
741638
Pseudomonas sp.
3-methoxybenzoate + NADH + 2 H+
-
-
-
?
3-nitrobenzaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
3-nitrobenzoate + NADH + 2 H+
-
-
-
?
4-carboxybenzaldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
4-carboxybenzoate + NADH + 2 H+
-
-
-
?
4-chlorobenzaldehyde + NAD+ + H2O
high activity
741638
Pseudomonas sp.
4-chlorobenzoate + NADH + 2 H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
4-hydroxybenzoate + NADH + 2 H+
-
-
-
?
4-methoxybenzaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
4-methoxybenzoate + NADH + 2 H+
-
-
-
?
4-nitrobenzaldehyde + NAD+ + H2O
moderate activity
741638
Pseudomonas sp.
4-nitrobenzoate + NADH + 2 H+
-
-
-
?
acetaldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
acetate + NADH + 2 H+
-
-
-
?
benzaldehyde + NAD+ + H2O
moderate to high activity
741638
Pseudomonas sp.
benzoate + NADH + 2 H+
-
-
-
?
butyraldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
butyrate + NADH + 2 H+
-
-
-
?
formaldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
formate + NADH + 2 H+
-
-
-
?
glutaraldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
glutarate + NADH + 2 H+
-
-
-
?
isovaleraldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
isovalerate + NADH + 2 H+
-
-
-
?
additional information
the enzyme shows maximum activity on salicylaldehyde and very poor activity on aliphatic aldehydes. The preferences of substrate (derivatives of benzaldehyde) for enzyme SALDH are dependent on nature, position and group of substituents. Enzyme SALDH prefers: (1) hydroxyl derivative at ortho and meta position, (2) chloro and nitro derivative at meta and para position, and (3) methoxy at meta position. The carboxyl group substitution is poorly preferred. Enzyme SALDH also shows activity on di-aromatic aldehydes. Substrate specificity, overview
741638
Pseudomonas sp.
?
-
-
-
-
propionaldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
propionate + NADH + 2 H+
-
-
-
?
salicylaldehyde + NAD+ + H2O
-
741638
Pseudomonas sp.
salicylate + NADH + 2 H+
-
-
-
?
salicylaldehyde + NAD+ + H2O
best substrate
741638
Pseudomonas sp.
salicylate + NADH + 2 H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotrimer
3 * 50000, SDS-PAGE
Pseudomonas sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
18
-
3-Hydroxybenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
26.4
-
benzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
59
-
4-nitrobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
65
-
3-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
93.6
-
Salicylaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
96
-
2-naphthaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
98
-
4-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
enzyme SALDH shows poor activity with NADP+
Pseudomonas sp.
NAD+
dependent on
Pseudomonas sp.
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0075
-
3-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.0773
-
4-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.126
-
4-nitrobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.136
-
2-naphthaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.185
-
Salicylaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.306
-
benzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
1.364
-
3-Hydroxybenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
enzyme SALDH shows poor activity with NADP+
Pseudomonas sp.
NAD+
dependent on
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
40% inhibition at 1 mM
Pseudomonas sp.
2,2'-dipyridyl
50% inhibition at 1 mM
Pseudomonas sp.
2-naphthaldehyde
substrate inhibition
Pseudomonas sp.
3-Chlorobenzaldehyde
substrate inhibition
Pseudomonas sp.
3-Hydroxybenzaldehyde
substrate inhibition
Pseudomonas sp.
4-Chlorobenzaldehyde
substrate inhibition
Pseudomonas sp.
4-nitrobenzaldehyde
substrate inhibition
Pseudomonas sp.
benzaldehyde
substrate inhibition
Pseudomonas sp.
EDTA
50% inhibition at 50 mM
Pseudomonas sp.
EGTA
60% inhibition at 50 mM
Pseudomonas sp.
Na+
inhibitory at higher concentration
Pseudomonas sp.
Salicylaldehyde
substrate inhibition, 86% and 70% decrease in activity at 0.05 mM and 0.1 mM, respectively
Pseudomonas sp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0075
-
3-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.0773
-
4-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.126
-
4-nitrobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.136
-
2-naphthaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.185
-
Salicylaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.306
-
benzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
1.364
-
3-Hydroxybenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics for NAD+
Pseudomonas sp.
0.001
-
benzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.0021
-
3-Hydroxybenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.0035
-
2-naphthaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.0039
-
3-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.0045
-
Salicylaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.0064
-
4-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.011
-
4-nitrobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
0.052
-
NAD+
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
the enzyme contains 1.8 mol/mol of enzyme
Pseudomonas sp.
Fe2+
the enzyme contains 0.4 mol/mol of enzyme
Pseudomonas sp.
K+
44% activating at 200 mM
Pseudomonas sp.
Mg2+
the enzyme contains 0.3 mol/mol of enzyme
Pseudomonas sp.
additional information
external addition of Ca2+, Mg2+, Fe2+ and Fe3+ at 10 mM, either as sulfate or chloride salts, fail to show an increase in the enzyme activity
Pseudomonas sp.
Zn2+
the enzyme contains 0.2 mol/mol of enzyme
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
150000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
salicylaldehyde + NAD+ + H2O
Pseudomonas sp.
-
salicylate + NADH + 2 H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 28fold from cellfree extract by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography, and gel filtration
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
36.3
-
purified native enzyme, pH 8.0, temperature not specified in the publication
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-naphthaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
1-naphthoate + NADH + 2 H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
2-carboxybenzoate + NADH + 2 H+
-
-
-
?
2-chlorobenzaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
2-chlorobenzoate + NADH + 2 H+
-
-
-
?
2-methoxybenzaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
2-methoxybenzoate + NADH + 2 H+
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
moderate activity
741638
Pseudomonas sp.
2-naphthoate + NADH + 2 H+
-
-
-
?
2-nitrobenzaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
2-nitrobenzoate + NADH + 2 H+
-
-
-
?
3-carboxybenzaldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
3-carboxybenzoate + NADH + 2 H+
-
-
-
?
3-chlorobenzaldehyde + NAD+ + H2O
moderate activity
741638
Pseudomonas sp.
3-chlorobenzoate + NADH + 2 H+
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
low to moderate activity
741638
Pseudomonas sp.
3-hydroxybenzoate + NADH + 2 H+
-
-
-
?
3-methoxybenzaldehyde + NAD+ + H2O
moderate activity
741638
Pseudomonas sp.
3-methoxybenzoate + NADH + 2 H+
-
-
-
?
3-nitrobenzaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
3-nitrobenzoate + NADH + 2 H+
-
-
-
?
4-carboxybenzaldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
4-carboxybenzoate + NADH + 2 H+
-
-
-
?
4-chlorobenzaldehyde + NAD+ + H2O
high activity
741638
Pseudomonas sp.
4-chlorobenzoate + NADH + 2 H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
4-hydroxybenzoate + NADH + 2 H+
-
-
-
?
4-methoxybenzaldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
4-methoxybenzoate + NADH + 2 H+
-
-
-
?
4-nitrobenzaldehyde + NAD+ + H2O
moderate activity
741638
Pseudomonas sp.
4-nitrobenzoate + NADH + 2 H+
-
-
-
?
acetaldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
acetate + NADH + 2 H+
-
-
-
?
benzaldehyde + NAD+ + H2O
moderate to high activity
741638
Pseudomonas sp.
benzoate + NADH + 2 H+
-
-
-
?
butyraldehyde + NAD+ + H2O
low activity
741638
Pseudomonas sp.
butyrate + NADH + 2 H+
-
-
-
?
formaldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
formate + NADH + 2 H+
-
-
-
?
glutaraldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
glutarate + NADH + 2 H+
-
-
-
?
isovaleraldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
isovalerate + NADH + 2 H+
-
-
-
?
additional information
the enzyme shows maximum activity on salicylaldehyde and very poor activity on aliphatic aldehydes. The preferences of substrate (derivatives of benzaldehyde) for enzyme SALDH are dependent on nature, position and group of substituents. Enzyme SALDH prefers: (1) hydroxyl derivative at ortho and meta position, (2) chloro and nitro derivative at meta and para position, and (3) methoxy at meta position. The carboxyl group substitution is poorly preferred. Enzyme SALDH also shows activity on di-aromatic aldehydes. Substrate specificity, overview
741638
Pseudomonas sp.
?
-
-
-
-
propionaldehyde + NAD+ + H2O
very low activity
741638
Pseudomonas sp.
propionate + NADH + 2 H+
-
-
-
?
salicylaldehyde + NAD+ + H2O
-
741638
Pseudomonas sp.
salicylate + NADH + 2 H+
-
-
-
?
salicylaldehyde + NAD+ + H2O
best substrate
741638
Pseudomonas sp.
salicylate + NADH + 2 H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotrimer
3 * 50000, SDS-PAGE
Pseudomonas sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
18
-
3-Hydroxybenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
26.4
-
benzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
59
-
4-nitrobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
65
-
3-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
93.6
-
Salicylaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
96
-
2-naphthaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
98
-
4-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas sp.
General Information
General Information
Commentary
Organism
physiological function
the enzyme catalyzes the oxidation of salicylaldehyde to salicylate in the carbaryl-degrading Pseudomonas sp. strain C6
Pseudomonas sp.
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme catalyzes the oxidation of salicylaldehyde to salicylate in the carbaryl-degrading Pseudomonas sp. strain C6
Pseudomonas sp.
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6000
-
4-nitrobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
8800
-
3-Hydroxybenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
12400
-
4-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
21500
-
3-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
23000
-
Salicylaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
26000
-
benzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
29000
-
2-naphthaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6000
-
4-nitrobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
8800
-
3-Hydroxybenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
12400
-
4-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
21500
-
3-Chlorobenzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
23000
-
Salicylaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
26000
-
benzaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
29000
-
2-naphthaldehyde
pH 8.0, temperature not specified in the publication
Pseudomonas sp.
Other publictions for EC 1.2.1.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743833
Jia
Evolutionary, computational, ...
Alteromonas naphthalenivorans
Sci. Rep.
7
43489
2017
-
-
1
-
4
-
1
9
-
-
1
1
-
4
-
-
1
1
-
-
-
-
2
2
-
1
-
8
1
1
-
1
4
-
-
-
-
1
1
-
4
-
-
1
4
9
-
-
1
1
-
-
-
1
-
-
-
-
2
2
-
1
-
8
1
1
-
-
1
5
5
1
8
8
741638
Singh
Purification and characteriza ...
Pseudomonas sp.
Appl. Biochem. Biotechnol.
172
806-819
2014
-
-
-
-
-
-
12
9
-
6
1
1
-
2
-
-
1
-
-
-
1
-
26
1
-
-
-
7
1
-
-
2
7
-
-
-
-
-
2
-
-
-
-
12
7
9
-
6
1
1
-
-
-
1
-
-
1
-
26
1
-
-
-
7
1
-
-
-
-
1
1
-
7
7
741793
Singh
Metabolic regulation and chro ...
Pseudomonas sp. C6
Arch. Microbiol.
195
521-535
2013
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
723872
Coitinho
Expression, purification and p ...
Pseudomonas putida, Pseudomonas putida G7
Acta Crystallogr. Sect. F
68
93-97
2012
-
-
1
1
-
-
-
-
-
-
1
-
-
11
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725888
Li
Physiological role of the nove ...
Pseudomonas putida, Pseudomonas putida ND6
Microbiol. Res.
166
643-653
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
3
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
4
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
696505
Nayak
Metabolism of acenaphthylene v ...
Stenotrophomonas sp. RMSK
Biodegradation
20
837-843
2009
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
2
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
2
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
691830
Zhao
-
A novel salicylaldehyde dehydr ...
Pseudomonas putida, Pseudomonas putida ND6
Chin. Sci. Bull.
52
1942-1948
2007
-
1
1
-
-
-
8
4
-
4
1
-
-
4
-
-
1
-
-
-
-
-
28
1
-
-
1
-
-
-
-
1
-
2
-
-
2
2
2
-
-
-
-
15
-
4
-
5
2
-
-
-
-
2
-
-
-
-
28
2
-
-
2
-
-
-
-
2
-
-
-
-
-
-
288315
Manohar
Degradation of naphthalene by ...
Pseudomonas sp., Pseudomonas sp. NGK1
Indian J. Exp. Biol.
33
353-356
1995
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288316
Eaton
Bacterial metabolism of naphth ...
Pseudomonas putida, Pseudomonas putida PpG1064
J. Bacteriol.
174
7542-7554
1992
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288317
Connors
Metabolism of naphthalene by p ...
Pseudomonas sp.
J. Bacteriol.
141
1052-1054
1980
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288318
Austen
-
Regulation of the plasmid-spec ...
Pseudomonas putida, Pseudomonas putida PpG7
J. Gen. Microbiol.
117
521-528
1980
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288319
Barnsley
Role and regulation of the ort ...
Pseudomonas putida, Pseudomonas putida PpG7, Pseudomonas sp.
J. Bacteriol.
125
404-408
1976
-
-
-
-
-
-
-
-
-
-
-
3
-
6
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288320
Barnsley
The induction of the enzymes o ...
Pseudomonas putida, Pseudomonas putida PpG7, Pseudomonas sp.
J. Gen. Microbiol.
88
193-196
1975
-
-
-
-
-
-
-
-
-
-
-
3
-
6
-
-
-
-
-
2
3
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288321
Shamsuzzaman
The regulation of naphthalene ...
Pseudomonas putida
Biochem. Biophys. Res. Commun.
60
582-589
1974
-
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-