BRENDA - Enzyme Database
show all sequences of 1.2.1.65

A novel salicylaldehyde dehydrogenase-NahV involved in catabolism of naphthalene from Pseudomonas putida ND6

Zhao, H.; Li, Y.; Chen, W.; Cai, B.; Chin. Sci. Bull. 52, 1942-1948 (2007)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
additional information
two genes code for salicylaldehyde dehydrogenase. NahF resides in the naphthalene degradation upper pathway operon as the meta-cleavage pathway gene, whereas NahV is situated outside of this operon. NahF-like genes occur in all naphthalene-degradation bacteria, whereas nahV-like genes are present in only some naphthalene-degrading bacteria; two genes code for salicylaldehyde dehydrogenase. NahF resides in the naphthalene degradation upper pathway operon as the meta-cleavage pathway gene, whereas NahV is situated outside of this operon. NahF-like genes occurr in all naphthalene-degradation bacteria, whereas nahV-like genes are present in only some naphthalene-degrading bacteria
Pseudomonas putida
Cloned(Commentary)
Cloned (Commentary)
Organism
nahF inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3); nahV inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3)
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
Al3+
inhibits NahF activity by 42%; inhibits NahV activity by 15%
Pseudomonas putida
Ba2+
inhibits NahF activity by 62%; inhibits NahV activity by 52%
Pseudomonas putida
Ca2+
inhibits NahF activity by 63%; inhibits NahV activity by 35%
Pseudomonas putida
Cs+
inhibits NahF activity by 44%; inhibits NahV activity by 69%
Pseudomonas putida
Cu2+
inhibits NahF activity by 70%
Pseudomonas putida
Li+
inhibits NahF activity by 50%; inhibits NahV activity by 57%
Pseudomonas putida
Mg2+
inhibits NahF activity by 34%; inhibits NahV activity by 36%
Pseudomonas putida
Ni2+
inhibits NahF activity by 60%; inhibits NahV activity by 70%
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00016
-
NAD+
-
Pseudomonas putida
0.00109
-
NAD+
-
Pseudomonas putida
0.034
-
Salicylaldehyde
-
Pseudomonas putida
0.146
-
Salicylaldehyde
-
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
enhances NahV activity by 63% activity
Pseudomonas putida
Fe2+
enhances NahF activity by 40% activity; enhances NahV activity by 152%
Pseudomonas putida
additional information
Zn2+ has no effect on NahF activity
Pseudomonas putida
Zn2+
enhances NahV activity by 14% activity
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
1 * 50000, SDS-PAGE; 1 * 50000, SDS-PAGE
Pseudomonas putida
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas putida
Q6XUJ4
-
-
Pseudomonas putida
Q6XUN9
-
-
Pseudomonas putida ND6
Q6XUJ4
-
-
Pseudomonas putida ND6
Q6XUN9
-
-
Purification (Commentary)
Purification (Commentary)
Organism
His-tagged NahF purified by Ni-NTA chromatography; His-tagged NahV purified by Ni-NTA chromatography
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-chlorosalicylaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
5-chlorosalicylate + NADH + H+
-
-
-
?
5-chlorosalicylaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida ND6
5-chlorosalicylate + NADH + H+
-
-
-
?
caprylic aldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
caprylic acid + NADH + H+
-
-
-
?
caprylic aldehyde + NAD+ + H2O
-
691830
Pseudomonas putida ND6
caprylic acid + NADH + H+
-
-
-
?
formaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
formate + NADH + H+
-
-
-
?
formaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida ND6
formate + NADH + H+
-
-
-
?
glutaraldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
glutarate + NADH + H+
-
-
-
?
glutaraldehyde + NAD+ + H2O
-
691830
Pseudomonas putida ND6
glutarate + NADH + H+
-
-
-
?
glyoxal + NAD+ + H2O
-
691830
Pseudomonas putida
glyoxylate + NADH + H+
-
-
-
?
glyoxal + NAD+ + H2O
-
691830
Pseudomonas putida ND6
glyoxylate + NADH + H+
-
-
-
?
m-nitrobenzaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
m-nitrobenzoate + NADH + H+
-
-
-
?
o-methoxybenxaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
o-methoxybenzoate + NADH + H+
-
-
-
?
o-nitrobenzaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
o-nitrobenzoate + NADH + H+
-
-
-
?
salicylaldehyde + NAD+ + H2O
best substrate
691830
Pseudomonas putida
salicylate + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 50000, SDS-PAGE; 1 * 50000, SDS-PAGE
Pseudomonas putida
Synonyms
Synonyms
Commentary
Organism
NahF
-
Pseudomonas putida
NahV
-
Pseudomonas putida
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
72.8% activity is retained for NahF after a 30 min exposure; NahV looses 88.8% activity after a 30 min exposure
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
;
Pseudomonas putida
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas putida
-
-
5.08
Pseudomonas putida
-
-
5.63
Application (protein specific)
Application
Commentary
Organism
additional information
two genes code for salicylaldehyde dehydrogenase. NahF resides in the naphthalene degradation upper pathway operon as the meta-cleavage pathway gene, whereas NahV is situated outside of this operon. NahF-like genes occur in all naphthalene-degradation bacteria, whereas nahV-like genes are present in only some naphthalene-degrading bacteria
Pseudomonas putida
additional information
two genes code for salicylaldehyde dehydrogenase. NahF resides in the naphthalene degradation upper pathway operon as the meta-cleavage pathway gene, whereas NahV is situated outside of this operon. NahF-like genes occurr in all naphthalene-degradation bacteria, whereas nahV-like genes are present in only some naphthalene-degrading bacteria
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
nahF inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3)
Pseudomonas putida
nahV inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3)
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Al3+
inhibits NahV activity by 15%
Pseudomonas putida
Al3+
inhibits NahF activity by 42%
Pseudomonas putida
Ba2+
inhibits NahV activity by 52%
Pseudomonas putida
Ba2+
inhibits NahF activity by 62%
Pseudomonas putida
Ca2+
inhibits NahV activity by 35%
Pseudomonas putida
Ca2+
inhibits NahF activity by 63%
Pseudomonas putida
Cs+
inhibits NahV activity by 69%
Pseudomonas putida
Cs+
inhibits NahF activity by 44%
Pseudomonas putida
Cu2+
inhibits NahF activity by 70%
Pseudomonas putida
Li+
inhibits NahV activity by 57%
Pseudomonas putida
Li+
inhibits NahF activity by 50%
Pseudomonas putida
Mg2+
inhibits NahV activity by 36%
Pseudomonas putida
Mg2+
inhibits NahF activity by 34%
Pseudomonas putida
Ni2+
inhibits NahV activity by 70%
Pseudomonas putida
Ni2+
inhibits NahF activity by 60%
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00016
-
NAD+
-
Pseudomonas putida
0.00109
-
NAD+
-
Pseudomonas putida
0.034
-
Salicylaldehyde
-
Pseudomonas putida
0.146
-
Salicylaldehyde
-
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
enhances NahV activity by 63% activity
Pseudomonas putida
Fe2+
enhances NahV activity by 152%
Pseudomonas putida
Fe2+
enhances NahF activity by 40% activity
Pseudomonas putida
additional information
Zn2+ has no effect on NahF activity
Pseudomonas putida
Zn2+
enhances NahV activity by 14% activity
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
1 * 50000, SDS-PAGE
Pseudomonas putida
Purification (Commentary) (protein specific)
Commentary
Organism
His-tagged NahF purified by Ni-NTA chromatography
Pseudomonas putida
His-tagged NahV purified by Ni-NTA chromatography
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-chlorosalicylaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
5-chlorosalicylate + NADH + H+
-
-
-
?
5-chlorosalicylaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida ND6
5-chlorosalicylate + NADH + H+
-
-
-
?
caprylic aldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
caprylic acid + NADH + H+
-
-
-
?
caprylic aldehyde + NAD+ + H2O
-
691830
Pseudomonas putida ND6
caprylic acid + NADH + H+
-
-
-
?
formaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
formate + NADH + H+
-
-
-
?
formaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida ND6
formate + NADH + H+
-
-
-
?
glutaraldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
glutarate + NADH + H+
-
-
-
?
glutaraldehyde + NAD+ + H2O
-
691830
Pseudomonas putida ND6
glutarate + NADH + H+
-
-
-
?
glyoxal + NAD+ + H2O
-
691830
Pseudomonas putida
glyoxylate + NADH + H+
-
-
-
?
glyoxal + NAD+ + H2O
-
691830
Pseudomonas putida ND6
glyoxylate + NADH + H+
-
-
-
?
m-nitrobenzaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
m-nitrobenzoate + NADH + H+
-
-
-
?
o-methoxybenxaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
o-methoxybenzoate + NADH + H+
-
-
-
?
o-nitrobenzaldehyde + NAD+ + H2O
-
691830
Pseudomonas putida
o-nitrobenzoate + NADH + H+
-
-
-
?
salicylaldehyde + NAD+ + H2O
best substrate
691830
Pseudomonas putida
salicylate + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 50000, SDS-PAGE
Pseudomonas putida
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
NahV looses 88.8% activity after a 30 min exposure
Pseudomonas putida
50
-
72.8% activity is retained for NahF after a 30 min exposure
Pseudomonas putida
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas putida
-
-
5.08
Pseudomonas putida
-
-
5.63
Other publictions for EC 1.2.1.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743833
Jia
Evolutionary, computational, ...
Alteromonas naphthalenivorans
Sci. Rep.
7
43489
2017
-
-
1
-
4
-
1
9
-
-
1
1
-
4
-
-
1
1
-
-
-
-
2
2
3
-
1
-
8
1
1
-
1
4
-
-
-
-
1
1
-
4
-
-
1
4
9
-
-
1
1
-
-
-
1
-
-
-
-
2
2
-
1
-
8
1
1
-
-
1
5
5
1
8
8
741638
Singh
Purification and characteriza ...
Pseudomonas sp.
Appl. Biochem. Biotechnol.
172
806-819
2014
-
-
-
-
-
-
12
9
-
6
1
1
-
2
-
-
1
-
-
-
1
-
26
1
2
-
-
-
7
1
-
-
2
7
-
-
-
-
-
2
-
-
-
-
12
7
9
-
6
1
1
-
-
-
1
-
-
1
-
26
1
-
-
-
7
1
-
-
-
-
1
1
-
7
7
741793
Singh
Metabolic regulation and chro ...
Pseudomonas sp. C6
Arch. Microbiol.
195
521-535
2013
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
723872
Coitinho
Expression, purification and p ...
Pseudomonas putida, Pseudomonas putida G7
Acta Crystallogr. Sect. F
68
93-97
2012
-
-
1
1
-
-
-
-
-
-
1
-
-
11
-
-
1
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
1
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1
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-
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-
1
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-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725888
Li
Physiological role of the nove ...
Pseudomonas putida, Pseudomonas putida ND6
Microbiol. Res.
166
643-653
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
3
-
4
-
3
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
3
-
4
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
696505
Nayak
Metabolism of acenaphthylene v ...
Stenotrophomonas sp. RMSK
Biodegradation
20
837-843
2009
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
2
-
2
-
1
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1
-
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1
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1
-
1
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-
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-
-
1
-
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-
1
-
-
2
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
691830
Zhao
-
A novel salicylaldehyde dehydr ...
Pseudomonas putida, Pseudomonas putida ND6
Chin. Sci. Bull.
52
1942-1948
2007
-
1
1
-
-
-
8
4
-
4
1
-
-
4
-
-
1
-
-
-
-
-
28
1
2
-
-
1
-
-
-
-
1
-
2
-
-
2
2
2
-
-
-
-
15
-
4
-
5
2
-
-
-
-
2
-
-
-
-
28
2
-
-
2
-
-
-
-
2
-
-
-
-
-
-
288315
Manohar
Degradation of naphthalene by ...
Pseudomonas sp., Pseudomonas sp. NGK1
Indian J. Exp. Biol.
33
353-356
1995
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
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-
-
1
1
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2
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1
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1
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-
2
-
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1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288316
Eaton
Bacterial metabolism of naphth ...
Pseudomonas putida, Pseudomonas putida PpG1064
J. Bacteriol.
174
7542-7554
1992
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
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-
1
1
-
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-
-
-
-
-
2
-
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-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288317
Connors
Metabolism of naphthalene by p ...
Pseudomonas sp.
J. Bacteriol.
141
1052-1054
1980
-
-
1
-
-
-
-
-
-
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-
1
-
1
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-
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1
2
-
1
-
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-
-
1
-
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-
1
1
-
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-
1
-
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1
2
-
1
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-
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-
-
-
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-
-
-
-
-
-
-
-
288318
Austen
-
Regulation of the plasmid-spec ...
Pseudomonas putida, Pseudomonas putida PpG7
J. Gen. Microbiol.
117
521-528
1980
-
-
-
-
-
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-
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2
-
5
-
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1
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2
-
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-
-
1
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1
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2
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1
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2
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-
-
-
-
-
-
-
-
288319
Barnsley
Role and regulation of the ort ...
Pseudomonas putida, Pseudomonas putida PpG7, Pseudomonas sp.
J. Bacteriol.
125
404-408
1976
-
-
-
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3
-
6
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2
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3
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2
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2
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3
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3
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288320
Barnsley
The induction of the enzymes o ...
Pseudomonas putida, Pseudomonas putida PpG7, Pseudomonas sp.
J. Gen. Microbiol.
88
193-196
1975
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3
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6
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3
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3
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288321
Shamsuzzaman
The regulation of naphthalene ...
Pseudomonas putida
Biochem. Biophys. Res. Commun.
60
582-589
1974
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