BRENDA - Enzyme Database
show all sequences of 1.2.1.60

Control of meta-cleavage degradation of 4-hydroxyphenylacetate in Pseudomonas putida

Barbour, M.G.; Bayly, R.C.; J. Bacteriol. 147, 844-850 (1981)

Data extracted from this reference:

General Stability
General Stability
Organism
heating at 50°C for 3 min and immediate chilling on ice abolishes enzyme activity in crude cell extracts
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
Pseudomonas putida
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
5-carboxymethyl-2-hydroxymuconate + NADH
-
Pseudomonas putida
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
Pseudomonas putida NCIB 9865
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
5-carboxymethyl-2-hydroxymuconate + NADH
-
Pseudomonas putida NCIB 9865
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida NCIB 9865
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
strict dependency for induction of enzyme synthesis of the meta-cleavage pathway by 4-hydroxyphenylacetate
Pseudomonas putida
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.07
-
-
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
288303
Pseudomonas putida
5-carboxymethyl-2-hydroxymuconate + NADH
-
288303
Pseudomonas putida
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
288303
Pseudomonas putida NCIB 9865
5-carboxymethyl-2-hydroxymuconate + NADH
-
288303
Pseudomonas putida NCIB 9865
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
General Stability (protein specific)
General Stability
Organism
heating at 50°C for 3 min and immediate chilling on ice abolishes enzyme activity in crude cell extracts
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
Pseudomonas putida
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
5-carboxymethyl-2-hydroxymuconate + NADH
-
Pseudomonas putida
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
Pseudomonas putida NCIB 9865
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
5-carboxymethyl-2-hydroxymuconate + NADH
-
Pseudomonas putida NCIB 9865
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
strict dependency for induction of enzyme synthesis of the meta-cleavage pathway by 4-hydroxyphenylacetate
Pseudomonas putida
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.07
-
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
288303
Pseudomonas putida
5-carboxymethyl-2-hydroxymuconate + NADH
-
288303
Pseudomonas putida
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
288303
Pseudomonas putida NCIB 9865
5-carboxymethyl-2-hydroxymuconate + NADH
-
288303
Pseudomonas putida NCIB 9865
?
Other publictions for EC 1.2.1.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
288297
Fawcett
5-Carboxymethyl-2-hydroxymucon ...
Escherichia coli, Escherichia coli C, Klebsiella pneumoniae, Klebsiella pneumoniae M5a1
FEMS Microbiol. Lett.
57
307-312
1989
-
-
-
-
-
-
-
4
-
-
4
4
-
19
-
-
2
-
-
2
3
-
4
2
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
4
4
-
-
-
2
-
2
3
-
4
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
288298
Alonso
-
Carboxymethylhydroxymuconic se ...
Pseudomonas putida
Biochem. Cell Biol.
64
1288-1293
1986
-
-
-
-
-
-
1
4
-
-
2
1
-
1
-
-
1
-
-
1
1
1
2
1
-
-
2
1
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
4
-
-
2
1
-
-
-
1
-
1
1
1
2
1
-
-
2
1
1
-
-
-
-
-
-
-
-
-
288299
Alonso
Kinetic properties of 5-carbox ...
Escherichia coli, Escherichia coli B / ATCC 11303
Biochimie
68
731-737
1986
-
-
-
-
-
-
2
2
-
-
-
2
-
178
-
-
1
-
-
1
-
-
2
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
-
-
-
2
-
-
-
1
-
1
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
288300
Alonso
Carboxymethylhydroxymuconic se ...
Escherichia coli
Biochim. Biophys. Acta
719
165-167
1982
-
-
-
-
-
-
3
3
-
-
2
1
-
2
-
-
1
-
-
1
1
-
2
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
3
-
-
2
1
-
-
-
1
-
1
1
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288303
Barbour
Control of meta-cleavage degra ...
Pseudomonas putida, Pseudomonas putida NCIB 9865
J. Bacteriol.
147
844-850
1981
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288301
Cooper
Catabolism of 3- and 4-hydroxy ...
Escherichia coli, Escherichia coli B / ATCC 11303, Escherichia coli C
J. Bacteriol.
143
302-306
1980
-
-
-
-
-
-
-
-
-
-
-
3
-
184
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288040
Blakley
The catabolism of L-tyrosine b ...
Arthrobacter globiformis, Arthrobacter globiformis PRL W15
Can. J. Microbiol.
23
1128-1139
1977
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-