BRENDA - Enzyme Database
show all sequences of 1.2.1.42

Characterization of a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8: a bacterial enzyme catalyzing the reduction of fatty acyl-CoA to fatty alcohol

Willis, R.M.; Wahlen, B.D.; Seefeldt, L.C.; Barney, B.M.; Biochemistry 50, 10550-10558 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli strain TB1
Marinobacter hydrocarbonoclasticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
hexadecanoyl-CoA
at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
0.05
-
cis-11-hexadecenal
at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
116000
-
x * 116000, SDS-PAGE
Marinobacter hydrocarbonoclasticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
arachidonoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
86% activity compared to hexadecanoyl-CoA
arachidonic aldehyde + CoA + NADP+
-
-
?
cis-11-hexadecenal + CoA + NADP+
Marinobacter hydrocarbonoclasticus
-
(E)-hexadec-11-enoyl-CoA + NADPh + H+
-
-
?
dodecanoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
26% activity compared to hexadecanoyl-CoA
dodecanal + CoA + NADP+
-
-
?
hexadecanoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
100% activity
1-hexadecanal + CoA + NADP+
-
-
?
additional information
Marinobacter hydrocarbonoclasticus
the enzyme exhibits no detectable reduction with free fatty acids, less than 1% activity with acetaldehyde and propanal
?
-
-
-
octadecanoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
69% activity compared to hexadecanoyl-CoA
octadecanal + CoA + NADP+
-
-
?
octanoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
9% activity compared to hexadecanoyl-CoA
octanal + CoA + NADP+
-
-
?
oleoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
78% activity compared to hexadecanoyl-CoA
oleic aldehyde + CoA + NADP+
-
-
?
palmitoleoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
99% activity compared to hexadecanoyl-CoA
palmitoleic aldehyde + CoA + NADP+
-
-
?
tetradecanoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
60% activity compared to hexadecanoyl-CoA
tetradecanal + CoA + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Marinobacter hydrocarbonoclasticus
-
-
-
Purification (Commentary)
Commentary
Organism
amylose column chromatography, metal affinity column chromatography, and G25 Sephadex gel filtration
Marinobacter hydrocarbonoclasticus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.015
-
using octanoyl-CoA as substrate, at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
0.034
-
using dodecanoyl-CoA as substrate, at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
0.04
-
using octadecanoyl-CoA as substrate, at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
0.044
-
using oleoyl-CoA as substrate, at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
0.049
-
using arachidonoyl-CoA as substrate, at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
0.057
-
using hexadecanoyl-CoA as substrate, at pH 7.0 and 37°C; using palmitoleyl-CoA as substrate, at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arachidonoyl-CoA + NADPH + H+
86% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
arachidonic aldehyde + CoA + NADP+
-
-
-
?
cis-11-hexadecenal + CoA + NADP+
-
724299
Marinobacter hydrocarbonoclasticus
(E)-hexadec-11-enoyl-CoA + NADPh + H+
-
-
-
?
dodecanoyl-CoA + NADPH + H+
26% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
dodecanal + CoA + NADP+
-
-
-
?
hexadecanoyl-CoA + NADPH + H+
100% activity
724299
Marinobacter hydrocarbonoclasticus
1-hexadecanal + CoA + NADP+
-
-
-
?
additional information
the enzyme exhibits no detectable reduction with free fatty acids, less than 1% activity with acetaldehyde and propanal
724299
Marinobacter hydrocarbonoclasticus
?
-
-
-
-
octadecanoyl-CoA + NADPH + H+
69% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
octadecanal + CoA + NADP+
-
-
-
?
octanoyl-CoA + NADPH + H+
9% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
octanal + CoA + NADP+
-
-
-
?
oleoyl-CoA + NADPH + H+
78% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
oleic aldehyde + CoA + NADP+
-
-
-
?
palmitoleoyl-CoA + NADPH + H+
99% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
palmitoleic aldehyde + CoA + NADP+
-
-
-
?
tetradecanoyl-CoA + NADPH + H+
60% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
tetradecanal + CoA + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 116000, SDS-PAGE
Marinobacter hydrocarbonoclasticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Marinobacter hydrocarbonoclasticus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
9
optimal activity is at pH 7.0, with activity falling dramatically below pH 6.5 and above 9.0
Marinobacter hydrocarbonoclasticus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Marinobacter hydrocarbonoclasticus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli strain TB1
Marinobacter hydrocarbonoclasticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Marinobacter hydrocarbonoclasticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
hexadecanoyl-CoA
at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
0.05
-
cis-11-hexadecenal
at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
116000
-
x * 116000, SDS-PAGE
Marinobacter hydrocarbonoclasticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
arachidonoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
86% activity compared to hexadecanoyl-CoA
arachidonic aldehyde + CoA + NADP+
-
-
?
cis-11-hexadecenal + CoA + NADP+
Marinobacter hydrocarbonoclasticus
-
(E)-hexadec-11-enoyl-CoA + NADPh + H+
-
-
?
dodecanoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
26% activity compared to hexadecanoyl-CoA
dodecanal + CoA + NADP+
-
-
?
hexadecanoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
100% activity
1-hexadecanal + CoA + NADP+
-
-
?
additional information
Marinobacter hydrocarbonoclasticus
the enzyme exhibits no detectable reduction with free fatty acids, less than 1% activity with acetaldehyde and propanal
?
-
-
-
octadecanoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
69% activity compared to hexadecanoyl-CoA
octadecanal + CoA + NADP+
-
-
?
octanoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
9% activity compared to hexadecanoyl-CoA
octanal + CoA + NADP+
-
-
?
oleoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
78% activity compared to hexadecanoyl-CoA
oleic aldehyde + CoA + NADP+
-
-
?
palmitoleoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
99% activity compared to hexadecanoyl-CoA
palmitoleic aldehyde + CoA + NADP+
-
-
?
tetradecanoyl-CoA + NADPH + H+
Marinobacter hydrocarbonoclasticus
60% activity compared to hexadecanoyl-CoA
tetradecanal + CoA + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
amylose column chromatography, metal affinity column chromatography, and G25 Sephadex gel filtration
Marinobacter hydrocarbonoclasticus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.015
-
using octanoyl-CoA as substrate, at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
0.034
-
using dodecanoyl-CoA as substrate, at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
0.04
-
using octadecanoyl-CoA as substrate, at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
0.044
-
using oleoyl-CoA as substrate, at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
0.049
-
using arachidonoyl-CoA as substrate, at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
0.057
-
using hexadecanoyl-CoA as substrate, at pH 7.0 and 37°C; using palmitoleyl-CoA as substrate, at pH 7.0 and 37°C
Marinobacter hydrocarbonoclasticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arachidonoyl-CoA + NADPH + H+
86% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
arachidonic aldehyde + CoA + NADP+
-
-
-
?
cis-11-hexadecenal + CoA + NADP+
-
724299
Marinobacter hydrocarbonoclasticus
(E)-hexadec-11-enoyl-CoA + NADPh + H+
-
-
-
?
dodecanoyl-CoA + NADPH + H+
26% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
dodecanal + CoA + NADP+
-
-
-
?
hexadecanoyl-CoA + NADPH + H+
100% activity
724299
Marinobacter hydrocarbonoclasticus
1-hexadecanal + CoA + NADP+
-
-
-
?
additional information
the enzyme exhibits no detectable reduction with free fatty acids, less than 1% activity with acetaldehyde and propanal
724299
Marinobacter hydrocarbonoclasticus
?
-
-
-
-
octadecanoyl-CoA + NADPH + H+
69% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
octadecanal + CoA + NADP+
-
-
-
?
octanoyl-CoA + NADPH + H+
9% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
octanal + CoA + NADP+
-
-
-
?
oleoyl-CoA + NADPH + H+
78% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
oleic aldehyde + CoA + NADP+
-
-
-
?
palmitoleoyl-CoA + NADPH + H+
99% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
palmitoleic aldehyde + CoA + NADP+
-
-
-
?
tetradecanoyl-CoA + NADPH + H+
60% activity compared to hexadecanoyl-CoA
724299
Marinobacter hydrocarbonoclasticus
tetradecanal + CoA + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 116000, SDS-PAGE
Marinobacter hydrocarbonoclasticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Marinobacter hydrocarbonoclasticus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
9
optimal activity is at pH 7.0, with activity falling dramatically below pH 6.5 and above 9.0
Marinobacter hydrocarbonoclasticus
Other publictions for EC 1.2.1.42
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741502
Doan
Biochemical characteristics o ...
Arabidopsis thaliana
Acta Biochim. Pol.
63
565-570
2016
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-
1
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1
1
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1
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2
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1
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2
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1
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1
1
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1
1
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1
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2
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1
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2
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3
3
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742372
Lan
-
Oxygen-tolerant coenzyme A-ac ...
Porphyromonas gingivalis
Energy Environ. Sci.
6
2672-2681
2013
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1
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2
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2
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1
1
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1
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3
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1
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1
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1
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1
1
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2
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1
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3
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1
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2
1
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2
2
724441
Doan
Biochemical characterization o ...
Arabidopsis thaliana
Biochim. Biophys. Acta
1821
1244-1255
2012
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1
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2
2
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5
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8
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1
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5
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1
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1
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1
1
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2
2
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5
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1
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5
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1
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724299
Willis
Characterization of a fatty ac ...
Marinobacter hydrocarbonoclasticus
Biochemistry
50
10550-10558
2011
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1
-
-
-
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2
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1
10
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2
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1
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6
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10
1
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1
1
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1
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1
1
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2
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1
10
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1
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6
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10
1
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1
1
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-
-
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724656
Hellenbrand
Fatty acyl-CoA reductases of b ...
Anser anser, Gallus gallus, Tyto alba
BMC Biochem.
12
64
2011
6
-
3
-
-
-
-
-
3
-
-
9
-
3
-
-
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-
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3
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9
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3
-
3
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3
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6
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3
3
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3
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9
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3
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9
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3
-
3
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-
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-
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720358
Teerawanichpan
Fatty acyl-CoA reductase and w ...
Euglena gracilis
Lipids
45
263-273
2010
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1
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-
-
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2
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3
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1
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699807
Doan
Functional expression of five ...
Arabidopsis thaliana
J. Plant Physiol.
166
787-796
2009
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1
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10
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9
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1
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3
3
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9
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288215
Ishige
Wax ester production from n-al ...
Acinetobacter
Appl. Environ. Microbiol.
68
1192-1195
2002
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1
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1
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1
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6
1
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1
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6
1
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288216
Metz
Purification of a jojoba embry ...
Simmondsia chinensis
Plant Physiol.
122
635-644
2000
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1
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2
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1
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1
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1
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1
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2
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1
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1
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1
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288212
Lee
Cysteine-286 as the site of ac ...
Photobacterium leiognathi, Photobacterium phosphoreum
Biochim. Biophys. Acta
1338
215-222
1997
-
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2
-
6
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6
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1
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2
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2
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2
2
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6
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1
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2
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288213
Vioque
Resolution and purification of ...
Pisum sativum
Arch. Biochem. Biophys.
340
64-72
1997
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4
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2
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4
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1
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2
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1
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1
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1
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4
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1
1
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288214
Reiser
Isolation of mutants of Acinet ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus BD413
J. Bacteriol.
179
2969-2975
1997
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1
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1
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6
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10
1
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1
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1
1
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1
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1
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3
1
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1
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