Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C247S | the mutant shows slightly reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
C253S | the mutation abolishes enzymatic activity | Arabidopsis thaliana |
C45S | the mutant shows stongly reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
I200G | the mutant also exhibits activity with NADP+ and shows decreased affinity for NAD+ compared to the wild type enzyme | Arabidopsis thaliana |
I200V | the mutant also exhibits activity with NADP+ and shows decreased affinity for NAD+ compared to the wild type enzyme | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
trans-2-nonenal | in 100 mM sodium diphosphate, at pH 8.0 and 22°C | Arabidopsis thaliana | |
0.005 | - |
dodecanal | in 100 mM sodium diphosphate, at pH 8.0 and 22°C | Arabidopsis thaliana | |
0.008 | - |
Nonanal | in 100 mM sodium diphosphate, at pH 8.0 and 22°C | Arabidopsis thaliana | |
0.029 | - |
octanal | in 100 mM sodium diphosphate, at pH 8.0 and 22°C | Arabidopsis thaliana | |
0.0403 | - |
4-hydroxynonenal | in 100 mM sodium diphosphate, at pH 8.0 and 22°C | Arabidopsis thaliana | |
0.071 | - |
hexanal | in 100 mM sodium diphosphate, at pH 8.0 and 22°C | Arabidopsis thaliana | |
0.119 | - |
NAD+ | wild type enzyme, with nonanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22°C | Arabidopsis thaliana | |
0.18 | - |
trans-2-hexenal | in 100 mM sodium diphosphate, at pH 8.0 and 22°C | Arabidopsis thaliana | |
0.421 | - |
NAD+ | wild type enzyme, with hexanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22°C | Arabidopsis thaliana | |
0.496 | - |
NAD+ | mutant enzyme I200V, with hexanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22°C | Arabidopsis thaliana | |
0.51 | - |
propionaldehyde | in 100 mM sodium diphosphate, at pH 8.0 and 22°C | Arabidopsis thaliana | |
3.218 | - |
NAD+ | mutant enzyme I200G, with hexanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isoform ALDH3H1 | Arabidopsis thaliana | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
2 * 56000, SDS-PAGE | Arabidopsis thaliana |
112000 | - |
gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Oxidation Stability | Organism |
---|---|
Oxidation leads to a decrease in enzymatic activities to less than 25-35% of the activity of the corresponding reduced form. Reduction of oxidized isoform ALDH3H1 after incubation with 10 mM dithiothreitol for 1 h results in a good recovery of activity to about 83% of the initial activity, but to only 44% after reduction with 10 mM GSH | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
His-tag binding column chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-2-nonenal + NAD+ + H2O | - |
Arabidopsis thaliana | (E)-non-2-enoate + NADH + H+ | - |
? | |
4-hydroxynonenal + NAD+ + H2O | - |
Arabidopsis thaliana | ? + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | - |
Arabidopsis thaliana | acetate + NADH + H+ | - |
? | |
dodecanal + NAD+ + H2O | - |
Arabidopsis thaliana | dodecanoate + NADH + H+ | - |
? | |
dodecanal + NAD+ + H2O | best substrate | Arabidopsis thaliana | dodecanoate + NADH + H+ | - |
? | |
hexanal + NAD+ + H2O | - |
Arabidopsis thaliana | hexanoate + NADH + H+ | - |
? | |
additional information | no activity with NADP+. Saturated aldehydes are preferred over unsaturated aldehydes, irrespective of chain length | Arabidopsis thaliana | ? | - |
? | |
nonanal + NAD+ + H2O | - |
Arabidopsis thaliana | nonanoate + NADH + H+ | - |
? | |
octanal + NAD+ + H2O | - |
Arabidopsis thaliana | octanoate + NADH + H+ | - |
? | |
propionaldehyde + NAD+ + H2O | - |
Arabidopsis thaliana | propanoate + NADH + H+ | - |
? | |
trans-2-hexenal + NAD+ + H2O | - |
Arabidopsis thaliana | (E)-hex-2-enoate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 56000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
ALDH3H1 | cytosolic isoform | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on, no activity with NADP+ | Arabidopsis thaliana |