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Literature summary for 1.2.1.12 extracted from
- Unfolded, oxidized, and thermoinactivated forms of glyceraldehyde-3-phosphate dehydrogenase interact with the chaperonin GroEL in different ways (2006), Biochim. Biophys. Acta, 1764, 831-838.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | - |
Oryctolagus cuniculus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
monomer, determined by SDS-PAGE | Oryctolagus cuniculus |
| 144000 | - |
tetramer, calculated | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Oryctolagus cuniculus | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GAPDH is subjected to gel filtration on a Sephadex G-100 column | Oryctolagus cuniculus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| GroEL-assisted reactivation after denaturation of GAPDH in the presence of guanidine hydrochloride | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Oryctolagus cuniculus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Oryctolagus cuniculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAPDH | - |
Oryctolagus cuniculus |
| glyceraldehyde-3-phosphate dehydrogenase | - |
Oryctolagus cuniculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
activity assay | Oryctolagus cuniculus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
100% thermoinactivation of GAPDH in the absence and in the presence of GroEL after 80 and 60 min, respectively, at 45°C | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
activity assay | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Oryctolagus cuniculus | |
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