Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme contains 8 SH groups per tetramer of which 4 are highly reactive and essential for activity | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Bacillus cereus | |
iodoacetate | - |
Bacillus cereus | |
Na2S4O6 | - |
Bacillus cereus | |
NEM | - |
Bacillus cereus | |
Sodium tetrathionate | - |
Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
NAD+ | - |
Bacillus cereus | |
0.51 | - |
D-glyceraldehyde 3-phosphate | - |
Bacillus cereus | |
1 | - |
arsenate | - |
Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
high content of beta-structure, 45% | Bacillus cereus |
36000 | - |
4 * 36000, SDS-PAGE | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus cereus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
vegetative | - |
Bacillus cereus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Bacillus cereus | 3-phospho-D-glyceroyl arsenate + NADH + H+ | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Bacillus cereus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 36000, SDS-PAGE | Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.5, 60 min, stable | Bacillus cereus |
60 | - |
pH 7.5, 30 min, about 40% loss of activity | Bacillus cereus |
70 | - |
loss of activity within a few min | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | pH 7.0: about 70% of maximal activity, pH 9.5: about 55% of maximal activity | Bacillus cereus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus cereus |