Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36600 | - |
2 * 36600, calculated from sequence | Sulfurisphaera tokodaii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced ferredoxin + NADP+ | Sulfurisphaera tokodaii | the enzyme plays an important role in the redox cycle of ferredoxin in the archaeon | 2 oxidized ferredoxin + NADPH + H+ | - |
r | |
2 reduced ferredoxin + NADP+ | Sulfurisphaera tokodaii 7 | the enzyme plays an important role in the redox cycle of ferredoxin in the archaeon | 2 oxidized ferredoxin + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | Q96YN9 | - |
- |
Sulfurisphaera tokodaii 7 | Q96YN9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced ferredoxin + NADP+ | the enzyme plays an important role in the redox cycle of ferredoxin in the archaeon | Sulfurisphaera tokodaii | 2 oxidized ferredoxin + NADPH + H+ | - |
r | |
2 reduced ferredoxin + NADP+ | high specificity for NADPH, the activity with NADH is hardly observed without the addition of an external flavin | Sulfurisphaera tokodaii | 2 oxidized ferredoxin + NADPH + H+ | - |
r | |
2 reduced ferredoxin + NADP+ | the enzyme plays an important role in the redox cycle of ferredoxin in the archaeon | Sulfurisphaera tokodaii 7 | 2 oxidized ferredoxin + NADPH + H+ | - |
r | |
2 reduced ferredoxin + NADP+ | high specificity for NADPH, the activity with NADH is hardly observed without the addition of an external flavin | Sulfurisphaera tokodaii 7 | 2 oxidized ferredoxin + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 36600, calculated from sequence | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
ST2133 | locus name | Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Sulfurisphaera tokodaii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 0.46 mol FAD/mol subunit | Sulfurisphaera tokodaii | |
FMN | activates. Vmax is 1.38 and 21.8 U/mg (at 70°C) in the absence and presence of 1 mM FMN | Sulfurisphaera tokodaii |