Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Synechocystis sp. |
60000 | - |
gel filtration | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | Q55318 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.31 | - |
substrate Fe(III)-EDTA, pH 7.0, 25°C | Synechocystis sp. |
0.39 | - |
substrate ferricytochrome c, pH 7.0, 25°C | Synechocystis sp. |
5.92 | - |
substrate FMN, pH 7.0, 25°C | Synechocystis sp. |
6 | - |
substrate FAD, pH 7.0, 25°C | Synechocystis sp. |
35.27 | - |
substrate 2,6-dichlorophenolindophenol, pH 7.0, 25°C | Synechocystis sp. |
35.28 | - |
substrate ferricyanide, pH 7.0, 25°C | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADPH | - |
Synechocystis sp. | 2 ferrocyanide + NADP+ + H+ | - |
? | |
2 ferricytochrome c2 + NADPH | - |
Synechocystis sp. | 2 ferrocytochrome c2 + NADP+ + H+ | - |
? | |
FAD + NADPH + H+ | - |
Synechocystis sp. | reduced FAD + NADP+ | - |
? | |
Fe(III)-EDTA + NADPH + H+ | - |
Synechocystis sp. | Fe(II) + EDTA + NADP+ | - |
? | |
FMN + NADPH + H+ | - |
Synechocystis sp. | reduced FMN + NADP+ | - |
? | |
additional information | DNA degradation occurring in the presence of NADPH, Fe(III)-EDTA and hydrogen peroxide is potently enhanced by the purified enzyme. The enzyme is capable of functioning as ferric reductase and of driving the Fenton reaction in the absence or presence of free flavin | Synechocystis sp. | ? | - |
? | |
oxidized 2,6-dichlorophenolindophenol + NADPH + H+ | - |
Synechocystis sp. | reduced 2,6-dichlorophenolindophenol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34000, SDS-PAGE | Synechocystis sp. |
Synonyms | Comment | Organism |
---|---|---|
FNRS | - |
Synechocystis sp. |
PETH | - |
Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | and FMN, ratio of FAD to FMN is 7.5 to 1 | Synechocystis sp. | |
FMN | and FAD, ratio of FAD to FMN is 7.5 to 1 | Synechocystis sp. |