Cloned (Comment) | Organism |
---|---|
amplification from genomic DNA, overexpression as His6-tagged protein in Escherichia coli strain Tuner (DE3) | Clostridium acetobutylicum |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged enzyme, sitting-drop vapour-diffusion method, 20°C, 0.001 l of 14 mg/ml protein in 20 mM Tris-HCl, pH 7.0, is mixed with 0.001 ml of reservoir solution containing 35% v/v PEG 400, 0.1 M Tris-HCl, pH 8.5, and 0.15 M MgCl2, and equilibrated against 0.1 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.1 A resolution | Clostridium acetobutylicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium acetobutylicum | Q9AL95 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli to homogeneity | Clostridium acetobutylicum |
Synonyms | Comment | Organism |
---|---|---|
NADH:rubredoxin oxidoreductase | - |
Clostridium acetobutylicum |
NROR | - |
Clostridium acetobutylicum |
Organism | Comment | Expression |
---|---|---|
Clostridium acetobutylicum | NROR is an O2-inducible protein | up |
General Information | Comment | Organism |
---|---|---|
physiological function | NROR is a versatile electron donor for scavengers of O2 and reactive oxygen species | Clostridium acetobutylicum |