KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
Caffeine | pH 7.5, 35°C | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
1 * 90000 + 1 * 40000 + 1 * 20000, SDS-PAGE | Pseudomonas sp. |
40000 | - |
1 * 90000 + 1 * 40000 + 1 * 20000, SDS-PAGE | Pseudomonas sp. |
90000 | - |
1 * 90000 + 1 * 40000 + 1 * 20000, SDS-PAGE | Pseudomonas sp. |
158000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1530 | - |
pH 7.5, 35°C | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-80°C, 5 mM potassium phosphate buffer pH 7.5, enzyme loses activity after a few days | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
caffeine + ubiquinone-0 | preferred electron acceptor | Pseudomonas sp. | trimethyl uric acid + ubiquinol-0 | the oxygen atom in trimethyl uric acid is derived exclusively from water | ? | |
additional information | enzyme is specific for caffeine and theobromine. No activity with xanthine, 3-methylxanthine, 7-methylxanthine, trimethyl uric acid, and 3,7-dimethyl uric acid | Pseudomonas sp. | ? | - |
? | |
theobromine + ubiquinone-0 | 46% of the activity with caffeine | Pseudomonas sp. | ? + ubiquinol-0 | - |
? | |
theophylline + ubiquinone-0 | 0.6% of the activity with caffeine | Pseudomonas sp. | ? + ubiquinol-0 | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 90000 + 1 * 40000 + 1 * 20000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
66 | - |
or above, activity increases linearly from 25 to 66°C, which is the highest temperature allowed by the experimental conditions | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
30 min, 77% residual activity | Pseudomonas sp. |
70 | - |
30 min, 11% residual activity | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.167 | - |
Caffeine | pH 7.5, 35°C | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
52% of maximum activity | Pseudomonas sp. |
8 | - |
47% of maximum activity | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is not NAD(P)+-dependent | Pseudomonas sp. | |
ubiquinone-0 | preferred electron acceptor | Pseudomonas sp. |