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Literature summary for 1.17.4.1 extracted from

  • Torrents, E.; Westman, M.; Sahlin, M.; Sjoeberg, B.M.
    Ribonucleotide reductase modularity: Atypical duplication of the ATP-cone domain in Pseudomonas aeruginosa (2006), J. Biol. Chem., 281, 25287-25296.
    View publication on PubMed
Search for more literature for 1.17.4.1

Activating Compound

Activating Compound Comment Organism Structure
dATP activation at low concentration with a KL1 value for specificity site binding of 0.0032 mM, inhibition at higher concentration with a KL2 value for activity site binding of 0.0173 mM Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information truncated NrdA lacking the N-terminal ATP-cone forms an NrdA2NrdB2 complex. Mutant protein is completely resistant to high concentrations of dATP Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
dATP activation at low concentration with a KL1 value for specificity site binding of 0.0032 mM, inhibition at higher concentration with a KL2 value for activity site binding of 0.0173 mM Pseudomonas aeruginosa
additional information not inhibitory: EDTA Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron is bound tightly to the protein. Enzyme activity is the same in presence and absence of EDTA Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 4 * 107000, subunit NrdA + 4 * 47000, subunit NrdB, SDS-PAGE Pseudomonas aeruginosa
107000
-
 4 * 107000, subunit NrdA + 4 * 47000, subunit NrdB, SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 enzyme is a complex of the NrdA protein harboring the active site and the allosteric sites and the NrdB protein harboring a tyrosyl radical
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
 pH 7.5, 25°C, anaerobic incubation and assay Pseudomonas aeruginosa
66
-
 pH 7.5, 25°C, anaerobic incubation, aerobic assay Pseudomonas aeruginosa
116
-
 pH 7.5, 25°C, aerobic incubation and assay Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP + reduced dithiothreitol
-
 Pseudomonas aeruginosa 2'-dCDP + oxidized dithiothreitol + H2O
-
 ?

Subunits

Subunits Comment Organism
More enzyme is a complex of the NrdA protein harboring the active site and the allosteric sites and the NrdB protein harboring a tyrosyl radical. Wild-type enzyme consists of four NrdA and four NrdB subunits. A truncated NrdA lacking the N-terminal ATP-cone forms an NrdA2NrdB2 complex Pseudomonas aeruginosa
octamer 4 * 107000, subunit NrdA + 4 * 47000, subunit NrdB, SDS-PAGE Pseudomonas aeruginosa