BRENDA - Enzyme Database
show all sequences of 1.16.8.1

Purification and characterization of cob(II)yrinic acid a,c-diamide reductase from Pseudomonas denitrificans

Blanche F.; Maton, L.; Debussche, L.; Thibaut, D.; J. Bacteriol. 174, 7452-7454 (1992)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0059
-
FMN
0.2 M Tris-HCl buffer, pH 8.0, 30C
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
16000
-
2 * 16000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
30000
-
gel permeation HPLC
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 cob(II)yrinic acid a,c-diamide + FMN
Pseudomonas denitrificans (nomen rejiciendum)
-
cob(I)yrinic acid a,c-diamide + FMNH2
-
-
?
cob(II)yrinic acid a,c-diamide + FMN
Pseudomonas denitrificans (nomen rejiciendum)
-
?
-
Pseudomonas denitrificans (nomen rejiciendum)
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas denitrificans (nomen rejiciendum)
-
-
-
Purification (Commentary)
Commentary
Organism
6,300-fold purification including Mono Q and FMN agarose chromatography
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 cob(II)yrinic acid a,c-diamide + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
cob(I)yrinic acid a,c-diamide + FMNH2
-
-
-
?
cob(II)alamin + FMN
41% activity compared to cob(II)yric acid
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(II)inamide + FMN
67-48% activity compared to cob(II)yric acid, percentage depends on method of activity detection
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(II)inamide phosphate + FMN
66% activity compared to cob(II)yric acid
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(II)yriC acid + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(II)yrinic acid a,c-diamide + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
cob(I)yrinic acid a,c-diamide + FMNH2
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(II)yrinic acid a,c-diamide + FMN
52-75% activity compared to cob(II)yric acid, percentage depends on method of activity detection
644281
Pseudomonas denitrificans (nomen rejiciendum)
cob(I)yrinic acid a,c-diamide + FMNH2
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(II)yrinic acid a,c-diamide + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
GDP-cob(II)inamide + FMN
71% activity compared to cob(II)yric acid
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 16000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0059
-
FMN
0.2 M Tris-HCl buffer, pH 8.0, 30C
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
16000
-
2 * 16000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
30000
-
gel permeation HPLC
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 cob(II)yrinic acid a,c-diamide + FMN
Pseudomonas denitrificans (nomen rejiciendum)
-
cob(I)yrinic acid a,c-diamide + FMNH2
-
-
?
cob(II)yrinic acid a,c-diamide + FMN
Pseudomonas denitrificans (nomen rejiciendum)
-
?
-
Pseudomonas denitrificans (nomen rejiciendum)
?
Purification (Commentary) (protein specific)
Commentary
Organism
6,300-fold purification including Mono Q and FMN agarose chromatography
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 cob(II)yrinic acid a,c-diamide + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
cob(I)yrinic acid a,c-diamide + FMNH2
-
-
-
?
cob(II)alamin + FMN
41% activity compared to cob(II)yric acid
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(II)inamide + FMN
67-48% activity compared to cob(II)yric acid, percentage depends on method of activity detection
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(II)inamide phosphate + FMN
66% activity compared to cob(II)yric acid
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(II)yriC acid + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(II)yrinic acid a,c-diamide + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
cob(I)yrinic acid a,c-diamide + FMNH2
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(II)yrinic acid a,c-diamide + FMN
52-75% activity compared to cob(II)yric acid, percentage depends on method of activity detection
644281
Pseudomonas denitrificans (nomen rejiciendum)
cob(I)yrinic acid a,c-diamide + FMNH2
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(II)yrinic acid a,c-diamide + FMN
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
GDP-cob(II)inamide + FMN
71% activity compared to cob(II)yric acid
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644281
Pseudomonas denitrificans (nomen rejiciendum)
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 16000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Other publictions for EC 1.16.8.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
644281
Blanche F.; Maton
Purification and characterizat ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
174
7452-7454
1992
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