Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M succinic acid-sodium phosphate dibasic-glycine buffer pH 4.0 and 18% (w/v) PEG 1500 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32400 | - |
x * 32400, His-tagged enzyme, SDS-PAGE | Escherichia coli |
32412 | - |
x * 32412, His-tagged enzyme, calculated from amino acid sequence | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA-Sepharose column chromatography and DEAE Sepharose column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an Fe(III)-siderophore + NADPH | - |
Escherichia coli | Fe(II) + an apo-siderophore + NADP+ + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32400, His-tagged enzyme, SDS-PAGE | Escherichia coli |
? | x * 32412, His-tagged enzyme, calculated from amino acid sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ferri-siderophore reductase | - |
Escherichia coli |
YqjH | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains FAD | Escherichia coli | |
NADPH | - |
Escherichia coli |