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Literature summary for 1.14.99.B10 extracted from

  • Agger, J.W.; Isaksen, T.; Várnai, A.; Vidal-Melgosa, S.; Willats, W.G.T.; Ludwig, R.; Horn, S.J.; Eijsink, V.G.H.; Westereng, B.
    Discovery of LPMO activity on hemicelluloses shows the importance of oxidative processes in plant cell wall degradation (2014), Proc. Natl. Acad. Sci. USA, 111, 6287-6292.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa Q7SHI8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barely beta-glucan + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O dominance of products increasing by three sugars in size, i.e., DP4, DP7, DP10, DP13, and so forth ?
glucomannan + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O release of clusters of products up to at least a degree of polymersation DP15, each cluster containing the keto-, gemdiol and native form of hexose oligosaccharides and acetylated species ?
lichenan + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O
-
 ?
additional information enzyme particularly acts on the glucose backbone of xyloglucan, accepting various substitutions (xylose, galactose) in almost all positions. It requires short stretches of contiguous (1->4)-beta-linked glucose units for activity. No substrates: (1->4)-beta-D-xylan, (1->4)-alpha-D-polygalacturonan, (1->4)-beta-D-mannan, (1->4)-beta-D-galactan, yeast beta-D-glucan, callose, and arabinoxylan Neurospora crassa ?
-
 ?
xyloglucan + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O two major oxidized products corresponding to the keto-form of single oxidized species, the most probable product configurations being GoxXXL and GoxXLL ?
xyloglucan 14-mer + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O LPMO9C cuts at the nonreducing end of the intermediate G-unit of a reduced 14-mer derived from tamarind xyloglucan mainly containing XXXGXXXGOH and produces GoxXXXGOH ?

Synonyms

Synonyms Comment Organism
LPMO9C
-
 Neurospora crassa