Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.99.69 extracted from

  • Carpentier, P.; Lepretre, C.; Basset, C.; Douki, T.; Torelli, S.; Duarte, V.; Hamdane, D.; Fontecave, M.; Atta, M.
    Structural, biochemical and functional analyses of tRNA-monooxygenase enzyme MiaE from Pseudomonas putida provide insights into tRNA/MiaE interaction (2020), Nucleic Acids Res., 48, 9918-9930 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.69

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment) Organism
structure by molecular replacement. MiaE consists of a catalytic diiron(III) domain with a four alpha-helix bundle fold. In the asymmetric unit, two MiaE monomers intertwine to form a homodimer. A narrow and very deep channel runs through the four-alpha-helix bundle from the surface of the protein to the diiron cluster Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the diiron active site is buried within the center of the four-alpha-helix bundle. The calculated Fe-Fe distances of 3.19 and 3.24 A are consistent with a diferric state Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q88KV1
-
-
Pseudomonas putida DSM6125 Q88KV1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenosine37 in tRNA + reduced acceptor + O2
-
 Pseudomonas putida N6-[(2E)-4-hydroxy-3-methylbut-2-en-1-yl]-2-(methylsulfanyl)adenosine37 in tRNA + acceptor + H2O
-
 ?
N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenosine37 in tRNA + reduced acceptor + O2
-
 Pseudomonas putida DSM6125 N6-[(2E)-4-hydroxy-3-methylbut-2-en-1-yl]-2-(methylsulfanyl)adenosine37 in tRNA + acceptor + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 24000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
miaE
-
 Pseudomonas putida
PP2188
-
 Pseudomonas putida