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Literature summary for 1.14.99.50 extracted from

  • Hu, W.; Song, H.; Sae Her, A.; Bak, D.W.; Naowarojna, N.; Elliott, S.J.; Qin, L.; Chen, X.; Liu, P.
    Bioinformatic and biochemical characterizations of C-S bond formation and cleavage enzymes in the fungus Neurospora crassa ergothioneine biosynthetic pathway (2014), Org. Lett., 16, 5382-5385 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.50

Application

Application Comment Organism
synthesis the ergothioneine biosynthetic pathway (EgtA-EgtE catalysis) provides an opportunity for ergothioneine production through metabolic engineering Mycolicibacterium smegmatis

Cloned(Commentary)

Cloned (Comment) Organism
contruction of an EgtB homologue sequence similarity network using a BLAST e value cutoff Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ non-heme iron, required for catalysis Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hercynine + gamma-L-glutamyl-L-cysteine + O2 Mycolicibacterium smegmatis
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 gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
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 ?
hercynine + gamma-L-glutamyl-L-cysteine + O2 Mycolicibacterium smegmatis ATCC 700084
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 gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
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 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0R5N0
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-
Mycolicibacterium smegmatis ATCC 700084 A0R5N0
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hercynine + gamma-L-glutamyl-L-cysteine + O2
-
 Mycolicibacterium smegmatis gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
hercynine + gamma-L-glutamyl-L-cysteine + O2
-
 Mycolicibacterium smegmatis ATCC 700084 gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
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 ?
additional information enzyme EgtB is extremely specific in terms of substrate specificity. Enzyme activity determination using : a 1H NMR assay of chemical shift of the imidazole hydrogen atoms Mycolicibacterium smegmatis ?
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 ?
additional information enzyme EgtB is extremely specific in terms of substrate specificity. Enzyme activity determination using : a 1H NMR assay of chemical shift of the imidazole hydrogen atoms Mycolicibacterium smegmatis ATCC 700084 ?
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 ?

Synonyms

Synonyms Comment Organism
EgtB
-
 Mycolicibacterium smegmatis
hercynine oxygenase UniProt Mycolicibacterium smegmatis

General Information

General Information Comment Organism
evolution OvoA, EC 1.14.99.52, and EgtB are related in sequence, while they are biochemically distinct Mycolicibacterium smegmatis
metabolism the mononuclear non-heme iron enzyme EgtB catalyzes an oxidative C-S bond formation in the ergothioneine biosynthesis. One of the EgtB substrates is gamma-Glu-Cys, which is part of the glutathione biosynthesis, resulting in competition between ergothioneine and glutathione biosyntheses Mycolicibacterium smegmatis