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Literature summary for 1.14.20.6 extracted from

  • Sun, Y.J.; He, J.M.; Kong, J.Q.
    Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq (2019), BMC Plant Biol., 19, 195 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.20.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Transetta (DE3) Albuca bracteata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47 1 dihydrokaempferol pH 6.0, 20°C Albuca bracteata
0.526
-
 dihydrokaempferol pH 6.0, 20°C Albuca bracteata

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity Albuca bracteata

Organism

Organism UniProt Comment Textmining
Albuca bracteata A0A482LTA5
-
-
Albuca bracteata A0A482LVU5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Albuca bracteata

Source Tissue

Source Tissue Comment Organism Textmining
bulb
-
 Albuca bracteata
-
flower
-
 Albuca bracteata
-
leaf high expression Albuca bracteata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-O-methyltaxifolin + 2-oxoglutarate + O2
-
 Albuca bracteata isorhamnetin + succinate + CO2 + H2O
-
 ?
dihydrokaempferol + 2-oxoglutarate + O2
-
 Albuca bracteata kaempferol + succinate + CO2 + H2O
-
 ?
dihydroquercetin + 2-oxoglutarate + O2
-
 Albuca bracteata quercetin + succinate + CO2 + H2O
-
 ?
pinobanksin + 2-oxoglutarate + O2
-
 Albuca bracteata galangin + succinate + CO2 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
FLS1
-
 Albuca bracteata
FLS2
-
 Albuca bracteata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
 Albuca bracteata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 37 0°C: about 60% of maximal activity, 37°C: about 85% of maximal activity Albuca bracteata
5 37 5°C: about 75% of maximal activity, 37°C: about 60% of maximal activity Albuca bracteata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Albuca bracteata

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 15% of maximal activity, pH 9.0: about 45% of maximal activity Albuca bracteata
5 8 pH 5.0: about 70% of maximal activity, pH 8.0: about 40% of maximal activity Albuca bracteata