Literature summary for 1.14.20.6 extracted from

Flores, G.; de la Pena Moreno, F.; Blanch, G.P.; del Castillo, M.L.
Phenylalanine ammonia-lyase, flavanone 3beta-hydroxylase and flavonol synthase enzyme activity by a new in vitro assay method in berry fruits (2014), Food Chem., 153, 130-133 .

Search for more literature for 1.14.20.6

Application

Application	Comment	Organism
analysis	an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods	Rubus plicatus
analysis	an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods	Rubus idaeus
analysis	an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods	Ribes nigrum
analysis	an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods	Fragaria x ananassa
analysis	an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods	Ribes rubrum

Organism

Organism	UniProt	Comment	Textmining
Fragaria x ananassa	Q07DT9	-	-
Ribes nigrum	-	-	-
Ribes rubrum	-	-	-
Rubus idaeus	-	-	-
Rubus plicatus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	-	Rubus plicatus	-
fruit	-	Rubus idaeus	-
fruit	-	Ribes nigrum	-
fruit	-	Fragaria x ananassa	-
fruit	-	Ribes rubrum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dihydrokaempferol + 2-oxoglutarate + O2	-	Rubus plicatus	kaempferol + succinate + CO2 + H2O	-	?
dihydrokaempferol + 2-oxoglutarate + O2	-	Rubus idaeus	kaempferol + succinate + CO2 + H2O	-	?
dihydrokaempferol + 2-oxoglutarate + O2	-	Ribes nigrum	kaempferol + succinate + CO2 + H2O	-	?
dihydrokaempferol + 2-oxoglutarate + O2	-	Fragaria x ananassa	kaempferol + succinate + CO2 + H2O	-	?
dihydrokaempferol + 2-oxoglutarate + O2	-	Ribes rubrum	kaempferol + succinate + CO2 + H2O	-	?

Synonyms

Synonyms	Comment	Organism
FLS	-	Rubus plicatus
FLS	-	Rubus idaeus
FLS	-	Ribes nigrum
FLS	-	Fragaria x ananassa
FLS	-	Ribes rubrum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Rubus plicatus
30	-	assay at	Rubus idaeus
30	-	assay at	Ribes nigrum
30	-	assay at	Fragaria x ananassa
30	-	assay at	Ribes rubrum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.3	-	assay at	Rubus plicatus
7.3	-	assay at	Rubus idaeus
7.3	-	assay at	Ribes nigrum
7.3	-	assay at	Fragaria x ananassa
7.3	-	assay at	Ribes rubrum

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Release 2023.1 (January 2023)