Application | Comment | Organism |
---|---|---|
synthesis | production of active flavonol synthase from Camellia sinensis in Escherichia coli, with high specific activity | Camellia sinensis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, His-tag | Camellia sinensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.139 | - |
dihydroquercetin | pH 7.5, 30°C | Camellia sinensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | presence of Fe2+ in purification buffer is required to yield active enzyme | Camellia sinensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camellia sinensis | A2TJG2 | - |
- |
Purification (Comment) | Organism |
---|---|
from inclusion bodies of recombinant Escherichia coli, denaturation with 6 M urea. Addition of FeSO4 to purification buffers significantly improves the recovery of active enzyme | Camellia sinensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroquercetin + 2-oxoglutarate + O2 | - |
Camellia sinensis | quercetin + succinate + CO2 + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
dihydroquercetin | pH 7.5, 30°C | Camellia sinensis |