Literature summary for 1.14.20.3 extracted from

Chang, W.; Guo, Y.; Wang, C.; Butch, S.; Rosenzweig, A.; Boal, A.; Krebs, C.; Bollinger Jr., J.
Mechanism of the C5 stereoinversion reaction in the biosynthesis of carbapenem antibiotics (2014), Science, 343, 1140-1144 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of CarC/Fe(II)/2-oxoglutarate complex	Pectobacterium carotovorum subsp. carotovorum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	Fe(II)-dependent oxygenase. An Fe(IV)-oxo intermediate abstracts the hydrogen (H) from C5, and tyrosine 165, a residue not visualized in the published structures of CarC lacking bound substrate, donates H to the opposite face of the resultant radical. The reaction oxidizes the Fe(II) cofactor to Fe(III), limiting wild-type CarC to one turnover, but substitution of the H-donating tyrosine disables stereoinversion and confers to CarC the capacity for catalytic substrate oxidation	Pectobacterium carotovorum subsp. carotovorum

Organism

Organism	UniProt	Comment	Textmining
Pectobacterium carotovorum subsp. carotovorum	Q9XB59	-	-

Synonyms

Synonyms	Comment	Organism
CarC	-	Pectobacterium carotovorum subsp. carotovorum

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Release 2023.1 (January 2023)