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Literature summary for 1.14.19.58 extracted from

  • Zhu, X.; De Laurentis, W.; Leang, K.; Herrmann, J.; Ihlefeld, K.; van Pee, K.H.; Naismith, J.H.
    Structural insights into regioselectivity in the enzymatic chlorination of tryptophan (2009), J. Mol. Biol., 391, 74-85.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.19.58

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged apoPyrH was expressed in Pseudomonas fluorescens BL915 Streptomyces rugosporus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor-diffusion method at 20°C, crystal structure of a tryptophan 5-halogenase (PyrH) bound to tryptophan and FAD Streptomyces rugosporus

Protein Variants

Protein Variants Comment Organism
E46D mutant enzyme folds properly Streptomyces rugosporus
E46Q mutant enzyme folds properly Streptomyces rugosporus
F49A/Y454F mutant enzyme is incorrectly folded Streptomyces rugosporus

Inhibitors

Inhibitors Comment Organism Structure
L-tryptophan substrate inhibition above 0.06 mM Streptomyces rugosporus

Organism

Organism UniProt Comment Textmining
Streptomyces rugosporus A4D0H5
-
-
Streptomyces rugosporus LL-42D005 A4D0H5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces rugosporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + FADH2 + Cl- + O2 + H+
-
 Streptomyces rugosporus 5-chloro-L-tryptophan + FAD + 2 H2O
-
 ?
L-tryptophan + FADH2 + Cl- + O2 + H+
-
 Streptomyces rugosporus LL-42D005 5-chloro-L-tryptophan + FAD + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
pyrH
-
 Streptomyces rugosporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces rugosporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Streptomyces rugosporus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01
-
 L-tryptophan mutant enzyme E46D, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C Streptomyces rugosporus
0.027
-
 L-tryptophan mutant enzyme F49A, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C Streptomyces rugosporus
0.039
-
 L-tryptophan mutant enzyme E46Q, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C Streptomyces rugosporus
0.05
-
 L-tryptophan mutant enzyme Y454F, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C Streptomyces rugosporus
0.54
-
 L-tryptophan wild type enzyme, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C Streptomyces rugosporus