Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NADH-cytochrome b5 reductase | EC 1.6.2.2, absolutely essential for DELTA6-desaturation | Rattus norvegicus | |
NADPH-cytochrome b5 reductase | EC 1.6.2.2, absolutely essential for DELTA6-desaturation | Rattus norvegicus | |
O2 | absolutely essential for DELTA6-desaturation | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bathophenanthroline sulfonate | mild inhibition | Rattus norvegicus | |
beta-mercaptoethanol | mild inhibition | Rattus norvegicus | |
dithiothreitol | mild inhibition | Rattus norvegicus | |
iron chelators | - |
Rattus norvegicus | |
KCN | - |
Rattus norvegicus | |
N-ethylmaleimide | only little effect | Rattus norvegicus | |
p-chloromercuribenzene sulfonate | - |
Rattus norvegicus | |
Tiron | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
linoleoyl-CoA | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | membrane | Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | DELTA6-desaturase contains 15.1 nmol iron/mg protein | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | 68000 | gel filtration | Rattus norvegicus |
66000 | - |
SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleic acid + AH2 + O2 | Rattus norvegicus | - |
gamma-linolenic acid + A + H2O | - |
? | |
linoleic acid + AH2 + O2 | Rattus norvegicus Wistar | - |
gamma-linolenic acid + A + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus Wistar | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phospholipoprotein | 20-40 M phospholipid per mol of protein | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0344 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, stored under nitrogen the enzyme is unstable, loses 80% of its activity after repeated freezing and thawing | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleic acid + AH2 + O2 | - |
Rattus norvegicus | gamma-linolenic acid + A + H2O | - |
? | |
linoleic acid + AH2 + O2 | - |
Rattus norvegicus Wistar | gamma-linolenic acid + A + H2O | - |
? | |
linoleoyl-CoA + AH2 + O2 | - |
Rattus norvegicus | gamma-linolenoyl-CoA + A + 2 H2O | - |
? | |
linoleoyl-CoA + AH2 + O2 | - |
Rattus norvegicus Wistar | gamma-linolenoyl-CoA + A + 2 H2O | - |
? | |
octadeca-9,12-dienoic acid + AH2 + O2 | - |
Rattus norvegicus | gamma-linolenic acid + A + H2O | - |
? | |
octadeca-9,12-dienoic acid + AH2 + O2 | - |
Rattus norvegicus Wistar | gamma-linolenic acid + A + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | absolutely essential for DELTA6-desaturation | Rattus norvegicus | |
linoleoyl-CoA | absolutely essential for DELTA6-desaturation | Rattus norvegicus | |
NADH | absolutely essential for DELTA6-desaturation | Rattus norvegicus | |
NADPH | absolutely essential for DELTA6-desaturation | Rattus norvegicus |