BRENDA - Enzyme Database show
show all sequences of 1.14.19.26

A bifunctional delta-fatty acyl acetylenase/desaturase from the moss Ceratodon purpureus. A new member of the cytochrome b5 superfamily

Sperling, P.; Lee, M.; Girke, T.; Zaehringer, U.; Stymne, S.; Heinz, E.; Eur. J. Biochem. 267, 3801-3811 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain InvSc1
Ceratodon purpureus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-linolenic acid + ferrocytochrome b5 + O2 + H+
Ceratodon purpureus
-
stearidonic acid + ferricytochrome b5 + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ceratodon purpureus
A7UFH0
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenic acid + ferrocytochrome b5 + O2 + H+
-
733768
Ceratodon purpureus
stearidonic acid + ferricytochrome b5 + H2O
-
-
-
?
additional information
the fatty acids 16:3DELTA7,10,13, 20:2DELTA8,11, 20:3DELTA8,11,14 and 20:4DELTA5,8,11,14 do not serve as substrates
733768
Ceratodon purpureus
?
-
-
-
-
oleic acid + ferrocytochrome b5 + O2 + H+
-
733768
Ceratodon purpureus
?
-
-
-
?
palmitoleic acid + ferrocytochrome b5 + O2 + H+
-
733768
Ceratodon purpureus
?
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Ceratodon purpureus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain InvSc1
Ceratodon purpureus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Ceratodon purpureus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-linolenic acid + ferrocytochrome b5 + O2 + H+
Ceratodon purpureus
-
stearidonic acid + ferricytochrome b5 + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenic acid + ferrocytochrome b5 + O2 + H+
-
733768
Ceratodon purpureus
stearidonic acid + ferricytochrome b5 + H2O
-
-
-
?
additional information
the fatty acids 16:3DELTA7,10,13, 20:2DELTA8,11, 20:3DELTA8,11,14 and 20:4DELTA5,8,11,14 do not serve as substrates
733768
Ceratodon purpureus
?
-
-
-
-
oleic acid + ferrocytochrome b5 + O2 + H+
-
733768
Ceratodon purpureus
?
-
-
-
?
palmitoleic acid + ferrocytochrome b5 + O2 + H+
-
733768
Ceratodon purpureus
?
-
-
-
?
Other publictions for EC 1.14.19.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733768
Sperling
A bifunctional delta-fatty acy ...
Ceratodon purpureus
Eur. J. Biochem.
267
3801-3811
2000
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438436
Cahoon
Redesign of soluble fatty acid ...
Thunbergia alata
Proc. Natl. Acad. Sci. USA
94
4872-4877
1997
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732022
Cahoon
DELTA6 Hexadecenoic acid is sy ...
Thunbergia alata
J. Biol. Chem.
269
27519-27526
1994
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1
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733420
Marra
Modulation of DELTA6 and DELTA ...
Rattus norvegicus
Biochim. Biophys. Acta
879
388-393
1986
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