Cloned (Comment) | Organism |
---|---|
DesA1 overexpressed in Escherichia coli BL21-(DE3) with the natural N-terminal methionine residue as the first amino acid | Mycobacterium tuberculosis |
DesA2 overexpressed in Escherichia coli BL21-(DE3) with the natural N-terminal methionine residue as the first amino acid | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
DesA2 crystals grown by the method of hanging drop vapor diffusion, one metal bound per subunit which is likely Mn2+, native and selenomethionine-substituted, 2.0 A resolution X-ray structure | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | absence of iron in both the native and selenomethionine-substituted crystals, X-ray fluorescence scan | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
unlabeled, purified DesA2 protein, MALDI-TOF, 31138 and 31345 m/z | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNZ5 | H37Rv,variant DesA2 | - |
Mycobacterium tuberculosis | P9WNZ7 | H37Rv, variants DesA1 | - |
Mycobacterium tuberculosis H37Rv | P9WNZ5 | H37Rv,variant DesA2 | - |
Mycobacterium tuberculosis H37Rv | P9WNZ7 | H37Rv, variants DesA1 | - |
Purification (Comment) | Organism |
---|---|
DesA2 isoform purified by ion exchange and gel filtration chromatographies | Mycobacterium tuberculosis |
Subunits | Comment | Organism |
---|---|---|
dimer | DesA2 isoform, gel filtration | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
acyl-ACP-desaturase | - |
Mycobacterium tuberculosis |
DesA1 | - |
Mycobacterium tuberculosis |
DesA2 | - |
Mycobacterium tuberculosis |