BRENDA - Enzyme Database show
show all sequences of 1.14.18.8

Effect of streptozotocin-induced diabetes on the activity of 7alpha-hydroxy-4-cholesten-3-one-specific 12alpha-hydroxylase in rats

Kimura, K.; Ogura, M.; Biol. Chem. Hoppe-Seyler 369, 1117-1120 (1988)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2
Rattus norvegicus
key enzyme for the formation of cholic acid
7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
activity in diabetic group is about half of the activity in the normal group
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2
-
658237
Rattus norvegicus
7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
-
-
?
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2
key enzyme for the formation of cholic acid
658237
Rattus norvegicus
7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2
Rattus norvegicus
key enzyme for the formation of cholic acid
7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
activity in diabetic group is about half of the activity in the normal group
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2
-
658237
Rattus norvegicus
7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
-
-
?
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2
key enzyme for the formation of cholic acid
658237
Rattus norvegicus
7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
-
-
?
Other publictions for EC 1.14.18.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
688997
Lee
Liver receptor homolog-1 regul ...
Mus musculus
Mol. Endocrinol.
22
1345-1356
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
700579
Liu
Dexamethasone exposure of neon ...
Rattus norvegicus
Pediatr. Res.
63
375-381
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
658153
Andersson
Rabbit liver contains one majo ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
1389
150-154
1998
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
2
-
-
1
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-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659131
Eggertsen
Molecular cloning and expressi ...
Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
271
32269-32275
1996
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
1
-
-
3
-
-
-
-
-
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-
-
3
-
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-
-
-
1
3
-
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-
-
-
-
-
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-
1
-
-
3
-
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-
-
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-
-
-
-
-
-
-
-
-
659123
Ishida
Purification and characterizat ...
Oryctolagus cuniculus
J. Biol. Chem.
267
21319-21323
1992
1
-
-
-
-
-
-
-
-
-
1
1
-
1
-
1
1
-
-
-
1
-
2
1
-
-
-
-
-
-
-
3
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
1
1
-
-
1
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658237
Kimura
Effect of streptozotocin-induc ...
Rattus norvegicus
Biol. Chem. Hoppe-Seyler
369
1117-1120
1988
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
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2
-
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-
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-
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1
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1
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1
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1
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1
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
660512
Noshiro
Assays for cholesterol 7alpha- ...
Rattus norvegicus
Steroids
45
539-550
1985
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
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1
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-
-
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-
1
1
-
2
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
659116
Murakami
Purification and characterizat ...
Oryctolagus cuniculus
J. Biol. Chem.
257
8030-8035
1982
1
-
-
-
-
1
4
1
1
-
1
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
1
-
-
2
-
-
1
-
4
-
1
1
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
1
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