Cloned (Comment) | Organism |
---|---|
generation of bi-allelic GGTA1 and CMAH double-knockout cells | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | production of alpha1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and cloning in porcine fetal fibroblast cells. Generation of bi-allelic GGTA1 and CMAH double-knockout cells, GGTA1/CMAH double-knockout (DKO) cells are cloned to produce piglets. Compared to cells from GGTA1-knockout pigs, human antibody binding and antibody-mediated complement-dependent cytotoxicity are significantly reduced in cells from GGTA1/CMAH DKO pigs, demonstrating that the pigs might exhibit reduced humoral rejection in xenotransplantation | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Sus scrofa | 9986 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+ | Sus scrofa | - |
CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
Chinese domestic miniature Wuzhishan pigs | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Sus scrofa | CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CMAH | - |
Sus scrofa |
cytidine monophosphate-N-acetylneuraminic acid hydroxylase | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Sus scrofa |
General Information | Comment | Organism |
---|---|---|
evolution | detailed phylogenetic analysis, overview | Sus scrofa |
physiological function | enzyme CMP-N-acetylneuraminic acid hydroxylase (CMAH) is responsible for the synthesis of N-glycolylneuraminic acid (Neu5Gc), a sialic acid present on the cell surface proteins of most deuterostomes. CMAH is the only enzyme capable of synthetizing Neu5Gc | Sus scrofa |