BRENDA - Enzyme Database
show all sequences of 1.14.18.2

Mouse liver cytidine-5-monophosphate-N-acetylneuraminic acid hydroxylase. Catalytic function and regulation

Shaw, L.; Schneckenburger, P.; Carlsen, J.; Christiansen, K.; Schauer, R.; Eur. J. Biochem. 206, 269-277 (1992)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
decyl glucoside
activation
Mus musculus
additional information
highest activity in 50 mM Hepes buffer, significant inhibition at increasing concentrations
Mus musculus
Nonidet P-40
effective inhibitor
Mus musculus
Octanoic acid
1 mM, modest activation
Mus musculus
octyl glucoside
effective inhibitor
Mus musculus
SDS
1 mM, modest activation
Mus musculus
Triton X-100
effective inhibitor
Mus musculus
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
5 mM, complete inhibition
Mus musculus
anti-(rat cytochrome b5) antiserum
-
Mus musculus
cardiolipin
3 mM, 15% inhibition
Mus musculus
CMP-N-glycoloylneuraminate
-
Mus musculus
ferrozine
0.7 mM, 82% inhibition
Mus musculus
KCN
5 mM, 49% inhibition
Mus musculus
Na2HPO4
2 mM, 86% inhibition
Mus musculus
Na4P2O7
2 mM, 54% loss of activity
Mus musculus
phosphatidic acid
3 mM, 20% inhibition
Mus musculus
phosphatidylinositol
3 mM, 68% inhibition
Mus musculus
Tiron
5 mM, 82% inhibition
Mus musculus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17000
-
gel filtration
Mus musculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CMP-N-acetylneuraminate + ferrocytochrome b5 + O2
the electron carrier cytochrome b5 is essential for activity
639334
Mus musculus
CMP-N-glycoloylneuraminate + ferricytrochrome b5 + H2O
-
-
-
?
CMP-N-acetylneuraminate + NADH + O2
-
639334
Mus musculus
CMP-N-glycoloylneuraminate + NAD+ + H2O
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.4
-
Mus musculus
Cofactor
Cofactor
Commentary
Organism
Structure
ferrocytochrome b5
the electron carrier cytochrome b5 is essential for activity
Mus musculus
NADH
-
Mus musculus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
decyl glucoside
activation
Mus musculus
additional information
highest activity in 50 mM Hepes buffer, significant inhibition at increasing concentrations
Mus musculus
Nonidet P-40
effective inhibitor
Mus musculus
Octanoic acid
1 mM, modest activation
Mus musculus
octyl glucoside
effective inhibitor
Mus musculus
SDS
1 mM, modest activation
Mus musculus
Triton X-100
effective inhibitor
Mus musculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ferrocytochrome b5
the electron carrier cytochrome b5 is essential for activity
Mus musculus
NADH
-
Mus musculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
5 mM, complete inhibition
Mus musculus
anti-(rat cytochrome b5) antiserum
-
Mus musculus
cardiolipin
3 mM, 15% inhibition
Mus musculus
CMP-N-glycoloylneuraminate
-
Mus musculus
ferrozine
0.7 mM, 82% inhibition
Mus musculus
KCN
5 mM, 49% inhibition
Mus musculus
Na2HPO4
2 mM, 86% inhibition
Mus musculus
Na4P2O7
2 mM, 54% loss of activity
Mus musculus
phosphatidic acid
3 mM, 20% inhibition
Mus musculus
phosphatidylinositol
3 mM, 68% inhibition
Mus musculus
Tiron
5 mM, 82% inhibition
Mus musculus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17000
-
gel filtration
Mus musculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CMP-N-acetylneuraminate + ferrocytochrome b5 + O2
the electron carrier cytochrome b5 is essential for activity
639334
Mus musculus
CMP-N-glycoloylneuraminate + ferricytrochrome b5 + H2O
-
-
-
?
CMP-N-acetylneuraminate + NADH + O2
-
639334
Mus musculus
CMP-N-glycoloylneuraminate + NAD+ + H2O
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.4
-
Mus musculus
Other publictions for EC 1.14.18.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745641
Gao
Production of alpha1,3-galact ...
Sus scrofa
J. Reprod. Dev.
63
17-26
2017
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1
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1
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2
2
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746206
Gandolfi
A novel variant in CMAH is as ...
Felis catus
PLoS ONE
11
e0154973
2016
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1
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1
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3
3
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746215
Omi
Molecular characterization of ...
Felis catus
PLoS ONE
11
e0165000
2016
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1
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8
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2
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1
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1
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2
2
-
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-
746539
Sakai
Knockout of cytidine monophos ...
Sus scrofa
Transplant. Proc.
48
1320-1322
2016
-
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1
-
1
-
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4
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1
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744458
Takahashi
Production and purification o ...
Chlorocebus aethiops, no activity in Homo sapiens
Biol. Pharm. Bull.
38
1220-1226
2015
-
1
1
-
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1
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3
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1
1
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1
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1
1
1
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1
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2
2
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727918
Bergfeld
Metabolism of vertebrate amino ...
Mus musculus
J. Biol. Chem.
287
28865-28881
2012
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1
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4
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1
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728834
Ikeda
A cloning of cytidine monophos ...
Sus scrofa
Transplant. Proc.
44
1136-1138
2012
-
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1
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6
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3
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711964
Kavaler
Pancreatic {beta}-cell failure ...
Mus musculus
FASEB J.
25
1887-1893
2011
-
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5
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1
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1
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1
1
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701238
Nystedt
Human CMP-N-acetylneuraminic a ...
Homo sapiens
Stem Cells
28
258-267
2010
-
1
1
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1
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1
1
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2
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1
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8
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1
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1
1
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1
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1
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8
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1
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-
711142
Song
Cloning and functional charact ...
Sus scrofa
Biochem. J.
427
179-188
2010
-
-
1
-
-
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-
-
-
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-
3
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6
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1
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1
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6
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1
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1
1
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-
712638
Tahara
Immunological property of anti ...
Mus musculus
J. Immunol.
184
3269-3275
2010
-
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1
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697129
Schauer
Low incidence of N-glycolylneu ...
no activity in Gallus gallus, no activity in Ornithorhynchus anatinus, no activity in Taeniopygia guttata
Carbohydr. Res.
344
1494-1500
2009
-
-
-
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13
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685822
Bighignoli
Cytidine monophospho-N-acetyln ...
Felis catus
BMC Genet.
8
27
2007
-
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1
-
5
-
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1
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1
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4
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1
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2
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1
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5
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2
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673862
Hayakawa
Fixation of the human-specific ...
no activity in Homo sapiens
Genetics
172
1139-1146
2006
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639332
Gollub
Isolation and characterization ...
Asterias rubens
Comp. Biochem. Physiol. B
134
89-101
2003
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1
3
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1
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5
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1
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1
1
2
2
1
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2
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1
2
2
1
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2
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658179
Chenu
Reduction of CMP-N-acetylneura ...
Cricetulus griseus
Biochim. Biophys. Acta
1622
133-144
2003
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5
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639323
Chou
Inactivation of CMP-N-acetylne ...
no activity in Homo sapiens
Proc. Natl. Acad. Sci. USA
99
11736-11741
2002
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6
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639336
Malykh
-
Regulation of N-glycolylneuram ...
Sus scrofa
Biochem. J.
22
2-29
2002
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639320
Martensen
Cloning and expression of a me ...
Asterias rubens
Eur. J. Biochem.
268
5157-5166
2001
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1
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1
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1
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7
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1
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1
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1
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639330
Malykh
Distribution and localization ...
Sus scrofa
Eur. J. Cell Biol.
80
48-58
2001
-
-
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1
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2
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5
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5
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672435
Malykh
N-Glycolylneuraminic acid in h ...
Homo sapiens
Biochimie
83
623-634
2001
-
1
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3
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1
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1
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639324
Irie
CMP-N-acetylneuraminic acid hy ...
Mus musculus, no activity in Homo sapiens
Biochem. Biophys. Res. Commun.
248
330-333
1998
-
-
1
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1
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4
-
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