General Stability | Organism |
---|---|
10 mM in the initial homogenization buffer is required for stability during 60-90 min incubation | Mus musculus |
10 mM in the initial homogenization buffer is required for stability during 60-90 min incubation | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-AMP | 5 mM, complete inhibition | Sus scrofa | |
3'-CMP | 5 mM, about 20% inhibition | Sus scrofa | |
3'-UMP | 5 mM, about 90% inhibition | Sus scrofa | |
5'-AMP | 5 mM, about 78% inhibition | Sus scrofa | |
5'-UMP | 5 mM, about 15% inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | 0.9 | CMP-N-acetylneuraminate | pH 7.4, 37°C, enzyme from NS-1 cells | Mus musculus | |
2.5 | - |
CMP-N-acetylneuraminate | pH 7.4, 37°C, enzyme from submaxillary gland | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Sus scrofa | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colonic mucosa | - |
Rattus norvegicus | - |
P3/NS1/1-Ag4.1 cell | - |
Mus musculus | - |
P3X63Ag8 cell | - |
Mus musculus | - |
submaxillary gland | - |
Sus scrofa | - |
submaxillary gland | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-70°C, stable in crude extract for several weeks | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity towards free or alpha-glycosidically bound N-acetylneuraminic acid | Mus musculus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 7.4 | enzyme preparation | Mus musculus |
6.4 | 7.4 | enzyme preparation | Sus scrofa |