Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzoic acid | effects of inhibitors on mushroom PPO are determined by using pyrogallol as substrate | Agaricus bisporus | |
L-cysteine | effects of inhibitors on mushroom PPO are determined by using pyrogallol as substrate | Agaricus bisporus | |
Sodium azide | effects of inhibitors on mushroom PPO are determined by using pyrogallol as substrate | Agaricus bisporus | |
Sodium fluoride | effects of inhibitors on mushroom PPO are determined by using pyrogallol as substrate | Agaricus bisporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
pyrocatechol | Lineweaver-Burk plot of pyrocatechol oxidation by the mushroom PPO | Agaricus bisporus | |
1.4 | - |
pyrogallol | Lineweaver-Burk plot of pyrogallol oxidation by the mushroom PPO | Agaricus bisporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | - |
Agaricus bisporus (J.E.Lange) Imbach | - |
Purification (Comment) | Organism |
---|---|
a 2fold purification in monophenolase and diphenolase activities is achieved by ammonium sulfate fractionation | Agaricus bisporus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the monophenolase activity is 3.35 EU/ml, and the diphenolase activity is 189.3 EU/ml. The specific acitivity after purification is 0.71 EU/mg for cresolase and 40.1 EU/mg for catecholase | Agaricus bisporus |
Storage Stability | Organism |
---|---|
PPO is relatively stable at -15°C for 44 days. | Agaricus bisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + O2 + AH2 | - |
Agaricus bisporus | L-dopa + H2O + A | - |
? | |
additional information | the enzyme shows low activity using mono- and triphenols as substrates but much greater activity with the diphenolic substrate | Agaricus bisporus | ? | - |
? | |
phenol + O2 + AH2 | - |
Agaricus bisporus | catechol + H2O + A | - |
? | |
phloroglucinol + 1/2 O2 | - |
Agaricus bisporus | ? | - |
? | |
pyrocatechol + 1/2 O2 | - |
Agaricus bisporus | 1,2-benzoquinone + H2O | - |
? | |
pyrogallol + 1/2 O2 | - |
Agaricus bisporus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
monophenol, o-diphenol:oxygen oxidoreductase | - |
Agaricus bisporus |
polyphenol oxidase | - |
Agaricus bisporus |
PPO | - |
Agaricus bisporus |
tyrosinase | a real tyrosinase because it shows both diphenolase and monophenolase activities | Agaricus bisporus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | determination of the temperature stability of mushroom PPO | Agaricus bisporus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
the enzyme is not very heat stable, and its activity decreases when incubated at the temperatures higher than 35°C | Agaricus bisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
PPO activity shows two pH optima, at 5.3 and 7.0 at 25°C when pyrogallol is used as the substrate | Agaricus bisporus |
7 | - |
PPO activity shows two pH optima, at 5.3 and 7.0 at 25°C when pyrogallol is used as the substrate | Agaricus bisporus |