KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM-value for the deoxicorticosterone 15beta-hydroxylase activity of recombinant BmCYP106A2 when using the NADPH/Anabaena ferredoxin electron donor system and different electron carrier proteins. Use of different AnFld mutants at positions W57, I59 and I92, which alter their aromatic and hydrophobic character, as electron donor to BmCYP106A2 | Priestia megaterium | |
0.00314 | - |
reduced ferredoxin | pH 7.4, 30°C, wild-type ferredoxin from the cyanobacterium Anabaena PCC 7119 | Priestia megaterium | |
0.00734 | - |
reduced flavodoxin | pH 7.4, 30°C, wild-type flavodoxin from the cyanobacterium Anabaena PCC 7119 | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | Q06069 | ATCC 13368, recombinant enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced ferredoxin + O2 | ferredoxin from the cyanobacterium Anabaena PCC 7119 | Priestia megaterium | 15beta-hydroxy-11-deoxycorticosterone + oxidized ferredoxin + H2O | - |
? | |
11-deoxycorticosterone + reduced flavodoxin + O2 | flavodoxin from the cyanobacterium Anabaena PCC 7119 | Priestia megaterium | 15beta-hydroxy-11-deoxycorticosterone + oxidized flavodoxin + H2O | - |
? | |
deoxycorticosterone + reduced ferredoxin + O2 | - |
Priestia megaterium | 15beta-hydroxy-11-deoxycorticosterone + oxidized ferredoxin + H2O | - |
r | |
deoxycorticosterone + reduced flavodoxin + O2 | - |
Priestia megaterium | 15beta-hydroxy-11-deoxycorticosterone + oxidized flavodoxin + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
BmCYP106A2 | - |
Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Priestia megaterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover-number for the deoxicorticosterone 15beta-hydroxylase activity of recombinant BmCYP106A2 when using the NADPH/Anabaena ferredoxin electron donor system and different electron carrier proteins. Use of different AnFld mutants at positions W57, I59 and I92, which alter their aromatic and hydrophobic character, as electron donor to BmCYP106A2 | Priestia megaterium | |
1.34 | - |
reduced flavodoxin | pH 7.4, 30°C, wild-type flavodoxin from the cyanobacterium Anabaena PCC 7119 | Priestia megaterium | |
3.3 | - |
reduced ferredoxin | pH 7.4, 30°C, wild-type ferredoxin from the cyanobacterium Anabaena PCC 7119 | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Priestia megaterium |