BRENDA - Enzyme Database
show all sequences of 1.14.15.8

Function and engineering of the 15beta-hydroxylase CYP106A2

Virus, C.; Lisurek, M.; Simgen, B.; Hannemann, F.; Bernhardt, R.; Biochem. Soc. Trans. 34, 1215-1218 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli, wild type and mutant S72A/V73I, the DELTA72 mutant, which lacks the first 72 amino acids, is not expressed in Escherichia coli at a detectable amount, suggesting that the truncated mutant cannot fold properly within the bacterial cell
Bacillus megaterium
Engineering
Protein Variants
Commentary
Organism
additional information
modelling of CYP106A2 and site-directed mutagenesis of the protein to check the accuracy of the computer-derived model of CYP106A2
Bacillus megaterium
S72A/V73I
mutant does not show a better stability in the crystallization process than the wild-type protein
Bacillus megaterium
General Stability
General Stability
Organism
by adding the ligands imidazole and metyrapone, it is not possible to prevent CYP106A2 from degradation in the crystallization process
Bacillus megaterium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47500
-
x * 47500
Bacillus megaterium
Organism
Organism
UniProt
Commentary
Textmining
Bacillus megaterium
Q06069
ATCC 13368
-
Storage Stability
Storage Stability
Organism
4°C, 4 weeks, degradation during storage
Bacillus megaterium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
the specificity of hydroxylation in beta-position can be altered by the choice of the electron transfer system. Replacing the natural electron transfer partners by peroxides, the ratio of 15alpha-/15beta-hydroxylation of progesterone is increased 1.3fold
696142
Bacillus megaterium
?
-
-
-
-
progesterone + reduced megaredoxin + O2
-
696142
Bacillus megaterium
15beta-hydroxyprogesterone + oxidized megaredoxin + H2O
-
-
-
?
testosterone + reduced adrenodoxin + O2
-
696142
Bacillus megaterium
15beta-hydroxytestosterone + oxidized adrenodoxin + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 47500
Bacillus megaterium
Synonyms
Synonyms
Commentary
Organism
15beta-hydroxylase
-
Bacillus megaterium
CYP106A2
-
Bacillus megaterium
Cofactor
Cofactor
Commentary
Organism
Structure
heme
-
Bacillus megaterium
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli, wild type and mutant S72A/V73I, the DELTA72 mutant, which lacks the first 72 amino acids, is not expressed in Escherichia coli at a detectable amount, suggesting that the truncated mutant cannot fold properly within the bacterial cell
Bacillus megaterium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
-
Bacillus megaterium
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
modelling of CYP106A2 and site-directed mutagenesis of the protein to check the accuracy of the computer-derived model of CYP106A2
Bacillus megaterium
S72A/V73I
mutant does not show a better stability in the crystallization process than the wild-type protein
Bacillus megaterium
General Stability (protein specific)
General Stability
Organism
by adding the ligands imidazole and metyrapone, it is not possible to prevent CYP106A2 from degradation in the crystallization process
Bacillus megaterium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47500
-
x * 47500
Bacillus megaterium
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 4 weeks, degradation during storage
Bacillus megaterium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
the specificity of hydroxylation in beta-position can be altered by the choice of the electron transfer system. Replacing the natural electron transfer partners by peroxides, the ratio of 15alpha-/15beta-hydroxylation of progesterone is increased 1.3fold
696142
Bacillus megaterium
?
-
-
-
-
progesterone + reduced megaredoxin + O2
-
696142
Bacillus megaterium
15beta-hydroxyprogesterone + oxidized megaredoxin + H2O
-
-
-
?
testosterone + reduced adrenodoxin + O2
-
696142
Bacillus megaterium
15beta-hydroxytestosterone + oxidized adrenodoxin + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 47500
Bacillus megaterium
Other publictions for EC 1.14.15.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745540
Kim
Crystal structure and functio ...
Bacillus sp. PAMC 23377
J. Microbiol. Biotechnol.
27
1472-1482
2017
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1
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1
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746507
Nikolaus
Engineering of CYP106A2 for s ...
Bacillus megaterium
Steroids
120
41-48
2017
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2
-
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1
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744706
Janocha
Crystal structure of CYP106A2 ...
Bacillus megaterium
ChemBioChem
17
852-860
2016
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1
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3
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744148
Kiss
Comparison of CYP106A1 and CY ...
Bacillus megaterium
Appl. Microbiol. Biotechnol.
99
8495-8514
2015
-
-
1
-
-
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4
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24
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2
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1
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2
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24
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745699
Schmitz
Steroid conversion with CYP10 ...
Bacillus megaterium
Microb. Cell Fact.
13
81
2014
-
1
1
-
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-
-
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-
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1
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6
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1
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6
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724061
Bleif
A new Bacillus megaterium whol ...
Bacillus megaterium, Bacillus megaterium ATCC 13368
Appl. Microbiol. Biotechnol.
93
1135-1146
2012
-
-
1
-
1
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1
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2
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6
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1
1
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1
2
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1
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6
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1
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1
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725584
Schumacher
Autodisplay of functional CYP1 ...
Bacillus megaterium
J. Biotechnol.
161
104-112
2012
-
-
1
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1
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1
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1
3
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4
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1
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1
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711747
Bleif
Identification of CYP106A2 as ...
Bacillus megaterium, Bacillus megaterium ATCC 13368
ChemBioChem
12
576-582
2011
-
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1
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2
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2
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4
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1
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4
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697322
Zehentgruber
Towards preparative scale ster ...
Bacillus megaterium
Chembiochem
11
713-721
2010
-
1
1
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696462
Goni
Cyanobacterial electron carrie ...
Bacillus megaterium
Biochim. Biophys. Acta
1794
1635-1642
2009
-
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3
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6
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1
1
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1
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2
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1
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3
1
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693956
Virus
Molecular evolution of a stero ...
Bacillus megaterium
Lipids
43
1133-1141
2008
-
-
1
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6
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5
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1
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1
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1
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6
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5
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1
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2
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1
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1
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697323
Lisurek
Theoretical and experimental e ...
Bacillus megaterium
Chembiochem
16
1439-1449
2008
-
-
1
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8
-
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16
-
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1
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1
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2
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1
1
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1
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8
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16
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1
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2
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1
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1
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699068
Ruijssenaars
Testosterone 15beta-hydroxylat ...
Bacillus megaterium
J. Biotechnol.
131
205-208
2007
-
1
1
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1
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696142
Virus
Function and engineering of th ...
Bacillus megaterium
Biochem. Soc. Trans.
34
1215-1218
2006
-
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1
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2
1
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1
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1
3
1
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695981
Lisurek
Identification of monohydroxy ...
Bacillus megaterium
Biochem. Biophys. Res. Commun.
319
677-682
2004
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1
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695980
Simgen
Substrate binding to 15beta-hy ...
Bacillus megaterium
Biochem. Biophys. Res. Commun.
269
737-742
2000
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700219
Rauschenbach
Cloning sequencing and express ...
Bacillus megaterium
Mol. Gen. Genet.
241
170-176
1993
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698684
Berg
Purification and characterizat ...
Bacillus megaterium
J. Biol. Chem.
254
5264-5271
1979
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698682
Berg
Characterization of a cytochro ...
Bacillus megaterium
J. Biol. Chem.
251
2831-2838
1976
2
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7
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