BRENDA - Enzyme Database
show all sequences of 1.14.15.8

Molecular evolution of a steroid hydroxylating cytochrome P450 using a versatile steroid detection system for screening

Virus, C.; Bernhardt, R.; Lipids 43, 1133-1141 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
CYP106A2 can be easily expressed in Escherichia coli with a high yield and can be reconstituted using the adrenal redox proteins, adrenodoxin and adrenodoxin reductase
Bacillus megaterium
Engineering
Protein Variants
Commentary
Organism
A106T
kcat/Km for conversion of 11-deoxycortisol is 3.17fold higher than wild-type value, 1.43fold increase in progesterone conversion
Bacillus megaterium
A106T/Q189K/T399S/R409L
kcat/Km for conversion of 11-deoxycortisol is 4.3fold higher than wild-type value, 1.13fold increase in progesterone conversion
Bacillus megaterium
A106T/R409L
kcat/Km for conversion of 11-deoxycortisol is 3fold higher than wild-type value, 1.43fold increase in progesterone conversion
Bacillus megaterium
D153V/I214F
kcat/Km for conversion of 11-deoxycortisol is 2.64fold higher than wild-type value, progesterone conversion is 93% of wild-type value
Bacillus megaterium
D217V
kcat/Km for conversion of 11-deoxycortisol is 2.68fold higher than wild-type value, 1.18fold increase in progesterone conversion
Bacillus megaterium
F165I
1.25fold increase in progesterone conversion
Bacillus megaterium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
11-deoxycortisol
pH 7.4, 30C, mutant A106T/Q189K/T399S/R409L, mutant D153V/I214F
Bacillus megaterium
0.059
-
11-deoxycortisol
pH 7.4, 30C, mutant A106T
Bacillus megaterium
0.066
-
11-deoxycortisol
pH 7.4, 30C, mutant D217V
Bacillus megaterium
0.068
-
11-deoxycortisol
pH 7.4, 30C, mutant A106T/R409L
Bacillus megaterium
0.093
-
11-deoxycortisol
pH 7.4, 30C, CYP106A2 wild-type enzyme
Bacillus megaterium
Organism
Organism
UniProt
Commentary
Textmining
Bacillus megaterium
Q06069
ATCC 13368
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Bacillus megaterium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
11-deoxycortisol + reduced adrenodoxin + O2
adrenodoxin is a [2Fe-2S] ferredoxin involved in electron transfer from NADPH+ (via a reductase) to cytochrome P-450 in the adrenal gland
693956
Bacillus megaterium
15beta-15,17,21-trihydroxypregn-4-ene-3,20-dione + oxidized adrenodoxin + H2O
-
-
-
?
progesterone + reduced adrenodoxin + O2
adrenodoxin is a [2Fe-2S] ferredoxin involved in electron transfer from NADPH+ (via a reductase) to cytochrome P-450 in the adrenal gland
693956
Bacillus megaterium
15beta-hydroxyprogesterone + oxidized adrenodoxin + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CYP106A2
-
Bacillus megaterium
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Bacillus megaterium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Bacillus megaterium
Cofactor
Cofactor
Commentary
Organism
Structure
adrenodoxin
-
Bacillus megaterium
NADPH
-
Bacillus megaterium
Cloned(Commentary) (protein specific)
Commentary
Organism
CYP106A2 can be easily expressed in Escherichia coli with a high yield and can be reconstituted using the adrenal redox proteins, adrenodoxin and adrenodoxin reductase
Bacillus megaterium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
adrenodoxin
-
Bacillus megaterium
NADPH
-
Bacillus megaterium
Engineering (protein specific)
Protein Variants
Commentary
Organism
A106T
kcat/Km for conversion of 11-deoxycortisol is 3.17fold higher than wild-type value, 1.43fold increase in progesterone conversion
Bacillus megaterium
A106T/Q189K/T399S/R409L
kcat/Km for conversion of 11-deoxycortisol is 4.3fold higher than wild-type value, 1.13fold increase in progesterone conversion
Bacillus megaterium
A106T/R409L
kcat/Km for conversion of 11-deoxycortisol is 3fold higher than wild-type value, 1.43fold increase in progesterone conversion
Bacillus megaterium
D153V/I214F
kcat/Km for conversion of 11-deoxycortisol is 2.64fold higher than wild-type value, progesterone conversion is 93% of wild-type value
Bacillus megaterium
D217V
kcat/Km for conversion of 11-deoxycortisol is 2.68fold higher than wild-type value, 1.18fold increase in progesterone conversion
Bacillus megaterium
F165I
1.25fold increase in progesterone conversion
Bacillus megaterium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
11-deoxycortisol
pH 7.4, 30C, mutant A106T/Q189K/T399S/R409L, mutant D153V/I214F
Bacillus megaterium
0.059
-
11-deoxycortisol
pH 7.4, 30C, mutant A106T
Bacillus megaterium
0.066
-
11-deoxycortisol
pH 7.4, 30C, mutant D217V
Bacillus megaterium
0.068
-
11-deoxycortisol
pH 7.4, 30C, mutant A106T/R409L
Bacillus megaterium
0.093
-
11-deoxycortisol
pH 7.4, 30C, CYP106A2 wild-type enzyme
Bacillus megaterium
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bacillus megaterium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
11-deoxycortisol + reduced adrenodoxin + O2
adrenodoxin is a [2Fe-2S] ferredoxin involved in electron transfer from NADPH+ (via a reductase) to cytochrome P-450 in the adrenal gland
693956
Bacillus megaterium
15beta-15,17,21-trihydroxypregn-4-ene-3,20-dione + oxidized adrenodoxin + H2O
-
-
-
?
progesterone + reduced adrenodoxin + O2
adrenodoxin is a [2Fe-2S] ferredoxin involved in electron transfer from NADPH+ (via a reductase) to cytochrome P-450 in the adrenal gland
693956
Bacillus megaterium
15beta-hydroxyprogesterone + oxidized adrenodoxin + H2O
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Bacillus megaterium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Bacillus megaterium
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745540
Kim
Crystal structure and functio ...
Bacillus sp. PAMC 23377
J. Microbiol. Biotechnol.
27
1472-1482
2017
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
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-
2
1
1
-
-
-
-
-
-
-
-
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-
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-
1
-
1
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746507
Nikolaus
Engineering of CYP106A2 for s ...
Bacillus megaterium
Steroids
120
41-48
2017
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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1
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2
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1
-
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-
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-
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-
-
-
-
744706
Janocha
Crystal structure of CYP106A2 ...
Bacillus megaterium
ChemBioChem
17
852-860
2016
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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3
-
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1
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1
1
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-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744148
Kiss
Comparison of CYP106A1 and CY ...
Bacillus megaterium
Appl. Microbiol. Biotechnol.
99
8495-8514
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
24
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
-
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-
-
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-
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-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
745699
Schmitz
Steroid conversion with CYP10 ...
Bacillus megaterium
Microb. Cell Fact.
13
81
2014
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
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-
-
-
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-
1
1
-
-
-
-
-
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6
-
-
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-
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-
-
-
-
-
-
-
-
-
724061
Bleif
A new Bacillus megaterium whol ...
Bacillus megaterium, Bacillus megaterium ATCC 13368
Appl. Microbiol. Biotechnol.
93
1135-1146
2012
-
-
1
-
1
-
-
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-
1
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-
2
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-
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6
-
1
1
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1
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2
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1
2
-
1
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1
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-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
725584
Schumacher
Autodisplay of functional CYP1 ...
Bacillus megaterium
J. Biotechnol.
161
104-112
2012
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
4
-
1
1
-
-
-
1
-
-
3
-
-
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-
1
3
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
711747
Bleif
Identification of CYP106A2 as ...
Bacillus megaterium, Bacillus megaterium ATCC 13368
ChemBioChem
12
576-582
2011
-
-
-
-
1
-
-
-
-
-
-
2
-
2
-
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-
-
-
-
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4
-
1
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1
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2
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-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697322
Zehentgruber
Towards preparative scale ster ...
Bacillus megaterium
Chembiochem
11
713-721
2010
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
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1
1
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-
-
-
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-
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-
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-
-
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-
696462
Goni
Cyanobacterial electron carrie ...
Bacillus megaterium
Biochim. Biophys. Acta
1794
1635-1642
2009
-
-
-
-
-
-
-
3
-
-
-
-
-
6
-
-
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-
-
-
-
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2
-
1
1
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3
1
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3
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2
-
1
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-
3
1
-
-
-
-
-
-
-
-
-
693956
Virus
Molecular evolution of a stero ...
Bacillus megaterium
Lipids
43
1133-1141
2008
-
-
1
-
6
-
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
1
1
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-
1
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2
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1
2
-
6
-
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5
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1
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-
2
-
1
-
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-
1
-
-
-
-
-
-
-
-
-
697323
Lisurek
Theoretical and experimental e ...
Bacillus megaterium
Chembiochem
16
1439-1449
2008
-
-
1
-
8
-
-
16
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
1
1
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1
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1
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-
8
-
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-
16
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1
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-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
699068
Ruijssenaars
Testosterone 15beta-hydroxylat ...
Bacillus megaterium
J. Biotechnol.
131
205-208
2007
-
1
1
-
1
-
-
-
-
-
-
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-
1
-
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1
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1
1
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1
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1
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-
696142
Virus
Function and engineering of th ...
Bacillus megaterium
Biochem. Soc. Trans.
34
1215-1218
2006
-
-
1
-
2
1
-
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-
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1
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3
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1
3
1
2
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1
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1
1
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2
1
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1
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1
3
1
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-
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695981
Lisurek
Identification of monohydroxy ...
Bacillus megaterium
Biochem. Biophys. Res. Commun.
319
677-682
2004
-
-
1
-
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1
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1
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1
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1
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1
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1
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695980
Simgen
Substrate binding to 15beta-hy ...
Bacillus megaterium
Biochem. Biophys. Res. Commun.
269
737-742
2000
-
-
1
-
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-
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1
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2
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1
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1
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1
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1
1
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1
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1
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1
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-
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-
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-
700219
Rauschenbach
Cloning sequencing and express ...
Bacillus megaterium
Mol. Gen. Genet.
241
170-176
1993
-
-
1
-
-
-
-
-
-
-
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-
6
-
-
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-
-
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1
-
2
-
-
-
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-
-
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1
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-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698684
Berg
Purification and characterizat ...
Bacillus megaterium
J. Biol. Chem.
254
5264-5271
1979
1
-
-
-
-
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-
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1
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1
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1
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1
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1
1
1
1
-
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1
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1
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1
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1
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1
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1
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1
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1
-
1
1
1
-
-
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1
-
-
1
-
-
-
-
-
-
698682
Berg
Characterization of a cytochro ...
Bacillus megaterium
J. Biol. Chem.
251
2831-2838
1976
2
-
-
-
-
-
8
7
-
-
1
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-
4
-
-
1
-
-
-
-
-
8
-
1
1
-
-
-
-
-
-
2
8
-
-
2
-
-
2
-
-
-
-
8
8
7
-
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1
-
-
-
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1
-
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-
-
8
-
1
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-
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