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Literature summary for 1.14.15.35 extracted from

  • Shafiee, A.; Hutchinson, C.R.
    Macrolide antibiotic biosynthesis: isolation and properties of two forms of 6-deoxyerythronolide B hydroxylase from Saccharopolyspora erythraea (Streptomyces erythreus) (1987), Biochemistry, 26, 6204-6210.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0115
-
(9R)-9-deoxo-9-hydroxy-6-deoxyerythronolide B pH 7.3, 35°C Saccharopolyspora erythraea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
PAGE Saccharopolyspora erythraea

Organism

Organism UniProt Comment Textmining
Saccharopolyspora erythraea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isolation of two forms, P-4501 and P-45011, by hydroxylapatite chromatography, having a P-450 content of 17.5 and 15.2 nmol/mg of protein, respectively Saccharopolyspora erythraea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9R)-9-deoxo-9-hydroxy-6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
Saccharopolyspora erythraea (9R)-9-deoxo-9-hydroxyerythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?
(9S)-9-deoxo-9-hydroxy-6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
Saccharopolyspora erythraea (9S)-9-deoxo-9-hydroxyerythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?
6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
Saccharopolyspora erythraea erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 44000, SDS-PAGE Saccharopolyspora erythraea

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 enzyme contains cytochrome P-450 in the low spin state with absorption maxima at 416, 532, and 565 nm, respectively Saccharopolyspora erythraea

pI Value

Organism Comment pI Value Maximum pI Value
Saccharopolyspora erythraea isoelectric focusing
-
4.6