KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0115 | - |
(9R)-9-deoxo-9-hydroxy-6-deoxyerythronolide B | pH 7.3, 35°C | Saccharopolyspora erythraea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
PAGE | Saccharopolyspora erythraea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora erythraea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isolation of two forms, P-4501 and P-45011, by hydroxylapatite chromatography, having a P-450 content of 17.5 and 15.2 nmol/mg of protein, respectively | Saccharopolyspora erythraea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9R)-9-deoxo-9-hydroxy-6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Saccharopolyspora erythraea | (9R)-9-deoxo-9-hydroxyerythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
(9S)-9-deoxo-9-hydroxy-6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Saccharopolyspora erythraea | (9S)-9-deoxo-9-hydroxyerythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Saccharopolyspora erythraea | erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000, SDS-PAGE | Saccharopolyspora erythraea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | enzyme contains cytochrome P-450 in the low spin state with absorption maxima at 416, 532, and 565 nm, respectively | Saccharopolyspora erythraea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharopolyspora erythraea | isoelectric focusing | - |
4.6 |