BRENDA - Enzyme Database
show all sequences of 1.14.15.28

A highly conserved mycobacterial cholesterol catabolic pathway

Garcia-Fernandez, E.; Frank, D.J.; Galan, B.; Kells, P.M.; Podust, L.M.; Garcia, J.L.; Ortiz de Montellano, P.R.; Environ. Microbiol. 15, 2342-2359 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli DH5alpha cells
Mycolicibacterium smegmatis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0103
-
cholest-4-en-3-one
at pH 7.5 and 37C
Mycolicibacterium smegmatis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
Mycolicibacterium smegmatis
-
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
-
?
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
Mycolicibacterium smegmatis mc(2)155
-
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycolicibacterium smegmatis
-
-
-
Mycolicibacterium smegmatis mc(2)155
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography, SP-Sepharose column chromatography, and Q-Sepharose column chromatography
Mycolicibacterium smegmatis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
-
738114
Mycolicibacterium smegmatis
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
-
-
?
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
-
738114
Mycolicibacterium smegmatis mc(2)155
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
heme
-
Mycolicibacterium smegmatis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli DH5alpha cells
Mycolicibacterium smegmatis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
-
Mycolicibacterium smegmatis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0103
-
cholest-4-en-3-one
at pH 7.5 and 37C
Mycolicibacterium smegmatis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
Mycolicibacterium smegmatis
-
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
-
?
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
Mycolicibacterium smegmatis mc(2)155
-
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography, SP-Sepharose column chromatography, and Q-Sepharose column chromatography
Mycolicibacterium smegmatis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
-
738114
Mycolicibacterium smegmatis
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
-
-
?
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
-
738114
Mycolicibacterium smegmatis mc(2)155
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
-
-
?
Other publictions for EC 1.14.15.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738629
Frank
Cholesterol ester oxidation by ...
Mycolicibacterium smegmatis
J. Biol. Chem.
289
30417-30425
2014
-
-
-
1
-
-
-
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738114
Garcia-Fernandez
A highly conserved mycobacteri ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc(2)155
Environ. Microbiol.
15
2342-2359
2013
-
-
1
-
-
-
-
1
-
-
-
2
-
11
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744481
Johnston
Substrate analog studies of t ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Bioorg. Med. Chem.
20
4064-4081
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
10
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
717811
Johnston
Functional redundancy of stero ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
285
36352-36360
2010
-
-
1
-
-
-
-
2
-
-
-
6
-
161
-
-
1
-
-
-
-
-
12
-
1
-
-
2
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
6
-
-
-
1
-
-
-
-
12
-
1
-
-
2
-
-
-
-
-
-
-
-
2
2
736407
Driscoll
Structural and biochemical cha ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618
J. Biol. Chem.
285
38270-38282
2010
-
-
1
1
-
2
-
-
-
-
-
2
-
6
-
-
1
4
-
-
-
-
6
-
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
3
-
-
1
1
-
-
-
717241
Ouellet
Reaction of Mycobacterium tube ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biochemistry
48
863-872
2009
-
-
1
-
-
-
1
-
-
-
-
-
-
161
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-