Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0103 | - |
cholest-4-en-3-one | at pH 7.5 and 37°C | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin | Mycolicibacterium smegmatis | - |
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O | - |
? | |
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin | Mycolicibacterium smegmatis mc(2)155 | - |
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Mycolicibacterium smegmatis mc(2)155 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, SP-Sepharose column chromatography, and Q-Sepharose column chromatography | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin | - |
Mycolicibacterium smegmatis | (25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O | - |
? | |
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin | - |
Mycolicibacterium smegmatis mc(2)155 | (25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP142A1 | isoform | Mycolicibacterium smegmatis |
CYP142A2 | isoform | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Mycolicibacterium smegmatis |