Literature summary for 1.14.15.1 extracted from

Murarka, V.C.; Batabyal, D.; Amaya, J.A.; Sevrioukova, I.F.; Poulos, T.L.
Unexpected differences between two closely related bacterial P450 camphor monooxygenases (2020), Biochemistry, 59, 2743-2750 .

Search for more literature for 1.14.15.1

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp. TCU-HL1	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-camphor + reduced putidaredoxin + O2	-	Pseudomonas sp. TCU-HL1	(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O	-	?

Synonyms

Synonyms	Comment	Organism
P450tcu	-	Pseudomonas sp. TCU-HL1

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
10.4	-	reduced putidaredoxin	redox partner is Pdxtcu, i.e native redox partner for isoform P450tcu, pH 7.4, 25°C	Pseudomonas sp. TCU-HL1
12.6	-	reduced putidaredoxin	redox partner is Pdx, i.e native redox partner for isoform P450cam, pH 7.4, 25°C	Pseudomonas sp. TCU-HL1

General Information

General Information	Comment	Organism
metabolism	camphor binding results in a low-spin to high-spin spectral change. The high-spin transition in P450cam occurs immediately upon camphor addition but is very slow with a half-life of 25 min. Association of camphor shows a DELTAH of <0. The product molecule can occupy two different orientations	Pseudomonas sp. TCU-HL1

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Release 2023.1 (January 2023)