Protein Variants | Comment | Organism |
---|---|---|
C330W | isoform CYP82E3 variant, active | Nicotiana tomentosiformis |
K269N | isoform CYP82E3 variant, no catalytic activity | Nicotiana tomentosiformis |
K269N/I274Null | isoform CYP82E3 variant, no catalytic activity | Nicotiana tomentosiformis |
K269N/I274Null/T279A | isoform CYP82E3 variant, no catalytic activity | Nicotiana tomentosiformis |
additional information | wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but conferrs no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to isentify regions and amino acids required for catalytic activity | Nicotiana tabacum |
additional information | wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but confers no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to identify regions and amino acids required for catalytic activity | Nicotiana tomentosiformis |
additional information | wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but confers no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to isentify regions and amino acids required for catalytic activity | Nicotiana tomentosiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0091 | - |
Nicotine | 23°C, pH 7.1 | Nicotiana tomentosiformis | |
0.0481 | - |
Nicotine | 23°C, pH 7.1 | Nicotiana tomentosiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
isoform CYP82E3 | - |
Nicotiana tomentosiformis | A1YJE2 | isoform CYP82E3 | - |
Nicotiana tomentosiformis | A1YJE3 | isoform CYP82E4 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | high expression in green leaf | Nicotiana tomentosiformis | - |
leaf | high induced during leaf senescence | Nicotiana tomentosiformis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Nicotiana tomentosiformis | nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |