Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.9 extracted from

  • Shen, X.; Zhou, D.; Lin, Y.; Wang, J.; Gao, S.; Kandavelu, P.; Zhang, H.; Zhang, R.; Wang, B.C.; Rose, J.; Yuan, Q.; Yan, Y.
    Structural insights into catalytic versatility of the flavin-dependent hydroxylase (HpaB) from Escherichia coli (2019), Sci. Rep., 9, 7087 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.9

Crystallization (Commentary)

Crystallization (Comment) Organism
the active site is covered by a loop of great plasticity and strong tolerance towards extensive mutagenesis, but also is flexible loop and enables the entrance and stable binding of substrates into the active site Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information changing the amino acid residues of section I of the flexible loop (mutant XS2, amino acids 207-211, GlyPheGlySerAla) into larger ones does not change the original secondary structure and the flexibility of the loop. The mutants show decreased activity towards substrates 4-coumaric acid, umbelliferone, resveratrol and naringenin. Changing the amino acid residues of section III of the flexible loop (mutant XS3, amino acids 215-217, GlyGluAsn) increases the Km values of towards p-coumaric acid and umbelliferone by 1.7fold and 1.2fold, respectively. The kcat value towards umbelliferone decreases by almost 2fold. Mutant XS4 incorporating the mutations in both XS2 and XS3 still keeps the secondary structure of the loop unchanged unchanged. In mutant XS5 the secondary structure of the loop is changed by replacing residues in section II of the loop (GlnValMet) with GlySerGly. Mutant XS5 shows reduced catalytic activity towards p-coumaric acid, umbelliferone and resveratrol. Mutant XS6 employs a loop containing mainly Gly, Ser, and Asp residues and shows the almost same specificity constant values towards p-coumaric acid and umbelliferone as wild-type Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.132
-
 4-coumaric acid mutant XS6, pH 7, 30°C Escherichia coli
0.138
-
 4-coumaric acid wild-type, pH 7, 30°C Escherichia coli
0.144
-
 resveratrol mutant XS6, pH 7, 30°C Escherichia coli
0.174
-
 resveratrol wild-type, pH 7, 30°C Escherichia coli
0.177
-
 umbelliferone mutant XS6, pH 7, 30°C Escherichia coli
0.192
-
 naringenin mutant XS6, pH 7, 30°C Escherichia coli
0.205
-
 umbelliferone mutant XS4, pH 7, 30°C Escherichia coli
0.211
-
 4-coumaric acid mutant XS4, pH 7, 30°C Escherichia coli
0.217
-
 umbelliferone wild-type, pH 7, 30°C Escherichia coli
0.235
-
 4-coumaric acid mutant XS5, pH 7, 30°C Escherichia coli
0.236
-
 resveratrol mutant XS3, pH 7, 30°C Escherichia coli
0.236
-
 4-coumaric acid mutant XS3, pH 7, 30°C Escherichia coli
0.266
-
 umbelliferone mutant XS3, pH 7, 30°C Escherichia coli
0.32
-
 resveratrol mutant XS5, pH 7, 30°C Escherichia coli
0.346
-
 umbelliferone mutant XS5, pH 7, 30°C Escherichia coli
0.35
-
 naringenin wild-type, pH 7, 30°C Escherichia coli
0.388
-
 4-coumaric acid mutant XS2, pH 7, 30°C Escherichia coli
0.405
-
 resveratrol mutant XS2, pH 7, 30°C Escherichia coli
0.417
-
 naringenin mutant XS3, pH 7, 30°C Escherichia coli
0.442
-
 resveratrol mutant XS4, pH 7, 30°C Escherichia coli
0.491
-
 umbelliferone mutant XS2, pH 7, 30°C Escherichia coli
0.628
-
 naringenin mutant XS4, pH 7, 30°C Escherichia coli
0.661
-
 naringenin mutant XS5, pH 7, 30°C Escherichia coli
1.062
-
 naringenin mutant XS2, pH 7, 30°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli A0A140NG21
-
-
Escherichia coli BL21-DE3 A0A140NG21
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaric acid + FADH2 + O2
-
 Escherichia coli caffeic acid + FAD + H2O
-
 ?
4-coumaric acid + FADH2 + O2
-
 Escherichia coli BL21-DE3 caffeic acid + FAD + H2O
-
 ?
naringenin + FADH2 + O2
-
 Escherichia coli ? + FAD + H2O
-
 ?
naringenin + FADH2 + O2
-
 Escherichia coli BL21-DE3 ? + FAD + H2O
-
 ?
resveratrol + FADH2 + O2
-
 Escherichia coli ? + FAD + H2O
-
 ?
resveratrol + FADH2 + O2
-
 Escherichia coli BL21-DE3 ? + FAD + H2O
-
 ?
umbelliferone + FADH2 + O2
-
 Escherichia coli ? + FAD + H2O
-
 ?
umbelliferone + FADH2 + O2
-
 Escherichia coli BL21-DE3 ? + FAD + H2O
-
 ?

Synonyms

Synonyms Comment Organism
HpaB
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.102
-
 naringenin mutant XS4, pH 7, 30°C Escherichia coli
0.108
-
 naringenin mutant XS2, pH 7, 30°C Escherichia coli
0.13
-
 naringenin mutant XS5, pH 7, 30°C Escherichia coli
0.15
-
 naringenin wild-type, pH 7, 30°C Escherichia coli
0.15
-
 naringenin mutant XS3, pH 7, 30°C Escherichia coli
0.15
-
 naringenin mutant XS6, pH 7, 30°C Escherichia coli
0.183
-
 4-coumaric acid mutant XS2, pH 7, 30°C Escherichia coli
0.217
-
 umbelliferone mutant XS5, pH 7, 30°C Escherichia coli
0.247
-
 umbelliferone mutant XS3, pH 7, 30°C Escherichia coli
0.282
-
 umbelliferone mutant XS2, pH 7, 30°C Escherichia coli
0.333
-
 resveratrol mutant XS5, pH 7, 30°C Escherichia coli
0.348
-
 umbelliferone mutant XS6, pH 7, 30°C Escherichia coli
0.365
-
 4-coumaric acid mutant XS6, pH 7, 30°C Escherichia coli
0.372
-
 umbelliferone mutant XS4, pH 7, 30°C Escherichia coli
0.375
-
 4-coumaric acid mutant XS5, pH 7, 30°C Escherichia coli
0.387
-
 4-coumaric acid wild-type, pH 7, 30°C Escherichia coli
0.405
-
 resveratrol mutant XS2, pH 7, 30°C Escherichia coli
0.417
-
 resveratrol mutant XS6, pH 7, 30°C Escherichia coli
0.418
-
 umbelliferone wild-type, pH 7, 30°C Escherichia coli
0.437
-
 resveratrol wild-type, pH 7, 30°C Escherichia coli
0.497
-
 4-coumaric acid mutant XS3, pH 7, 30°C Escherichia coli
0.512
-
 4-coumaric acid mutant XS4, pH 7, 30°C Escherichia coli
0.565
-
 resveratrol mutant XS3, pH 7, 30°C Escherichia coli
0.847
-
 resveratrol mutant XS4, pH 7, 30°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
 naringenin mutant XS2, pH 7, 30°C Escherichia coli
0.2
-
 naringenin mutant XS4, pH 7, 30°C Escherichia coli
0.2
-
 naringenin mutant XS5, pH 7, 30°C Escherichia coli
0.4
-
 naringenin wild-type, pH 7, 30°C Escherichia coli
0.4
-
 naringenin mutant XS3, pH 7, 30°C Escherichia coli
0.47
-
 4-coumaric acid mutant XS2, pH 7, 30°C Escherichia coli
0.6
-
 umbelliferone mutant XS2, pH 7, 30°C Escherichia coli
0.6
-
 umbelliferone mutant XS5, pH 7, 30°C Escherichia coli
0.8
-
 naringenin mutant XS6, pH 7, 30°C Escherichia coli
0.9
-
 umbelliferone mutant XS3, pH 7, 30°C Escherichia coli
1
-
 resveratrol mutant XS2, pH 7, 30°C Escherichia coli
1
-
 resveratrol mutant XS5, pH 7, 30°C Escherichia coli
1.56
-
 4-coumaric acid mutant XS5, pH 7, 30°C Escherichia coli
1.8
-
 umbelliferone mutant XS4, pH 7, 30°C Escherichia coli
1.9
-
 umbelliferone wild-type, pH 7, 30°C Escherichia coli
1.9
-
 resveratrol mutant XS4, pH 7, 30°C Escherichia coli
2
-
 umbelliferone mutant XS6, pH 7, 30°C Escherichia coli
2.1
-
 4-coumaric acid mutant XS3, pH 7, 30°C Escherichia coli
2.4
-
 resveratrol mutant XS3, pH 7, 30°C Escherichia coli
2.4
-
 4-coumaric acid mutant XS4, pH 7, 30°C Escherichia coli
2.5
-
 resveratrol wild-type, pH 7, 30°C Escherichia coli
2.8
-
 4-coumaric acid wild-type, pH 7, 30°C Escherichia coli
2.8
-
 4-coumaric acid mutant XS6, pH 7, 30°C Escherichia coli
2.9
-
 resveratrol mutant XS6, pH 7, 30°C Escherichia coli