Application | Comment | Organism |
---|---|---|
synthesis | enzyme shows relatively broad substrate specificity, which allows the conversion of a number of non-natural phenolic compounds, into the corresponding catechols. The reaction can be performed based on formate as the electron donor and the organometallic complex [Rh(bpy)-Cp*(H2O)]2+ (Cp*: 1,2,3,4,5-pentamethylcyclopentadiene, bpy: 2,2'-bipyridyl) as the catalyst for FAD reduction | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
in complex with FAD, to 1.94 A resolution | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H155A | drastically decreased hydroxylase activity with substrate 3-hydroxyphenylacetate | Escherichia coli |
I157A | complete loss of hydroxylase activity | Escherichia coli |
I157G | complete loss of hydroxylase activity | Escherichia coli |
I157S | complete loss of hydroxylase activity | Escherichia coli |
R113A | drastically decreased hydroxylase activity with substrate 3-hydroxyphenylacetate | Escherichia coli |
S210A | drastically decreased hydroxylase activity | Escherichia coli |
S210Q | drastically decreased hydroxylase activity | Escherichia coli |
V158G | complete loss of hydroxylase activity | Escherichia coli |
Y117A | drastically decreased hydroxylase activity with substrate 3-hydroxyphenylacetate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0094 | - |
3-hydroxyphenylacetate | pH 7.5, 23°C | Escherichia coli | |
0.0247 | - |
DL-4-hydroxymandelic acid | pH 7.5, 23°C | Escherichia coli | |
0.0339 | - |
2-(4-hydroxyphenyl)ethanol | pH 7.5, 23°C | Escherichia coli | |
0.0461 | - |
(3,4-dihydroxyphenyl)acetate | pH 7.5, 23°C | Escherichia coli | |
0.054 | - |
4-coumarate | pH 7.5, 23°C | Escherichia coli | |
0.253 | - |
phenol | pH 7.5, 23°C | Escherichia coli | |
0.515 | - |
methyl 4-hydroxybenzoate | pH 7.5, 23°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3,4-dihydroxyphenyl)acetate + NADH + H+ + O2 | - |
Escherichia coli | ? + NAD+ + H2O | - |
? | |
2-(4-hydroxyphenyl)ethanol + NADH + H+ + O2 | - |
Escherichia coli | ? + NAD+ + H2O | - |
? | |
3-hydroxyphenylacetate + NADH + H+ + O2 | - |
Escherichia coli | 3,4-hydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-coumarate + NADH + H+ + O2 | - |
Escherichia coli | ? + NAD+ + H2O | - |
? | |
DL-4-hydroxymandelic acid + NADH + H+ + O2 | - |
Escherichia coli | ? + NAD+ + H2O | - |
? | |
methyl 4-hydroxybenzoate + NADH + H+ + O2 | - |
Escherichia coli | ? + NAD+ + H2O | - |
? | |
additional information | no substrates: benzoic acid, dihydroxybenzoic acid, 4-methoxyphenylacetic acid, catechol, 4-hydroxy-D-2-phenylglycine, 4-hydroxy-L-2-phenylglycine, 3-hydroxy-4-methoxyphenylacetic acid, 4-tert-butylphenol, 3-methoxyphenylacetic acid, 4-hydroxy-3-methoxyphenylacetic acid | Escherichia coli | ? | - |
- |
|
phenol + NADH + H+ + O2 | - |
Escherichia coli | ? + NAD+ + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
methyl 4-hydroxybenzoate | pH 7.5, 23°C | Escherichia coli | |
0.3 | - |
phenol | pH 7.5, 23°C | Escherichia coli | |
0.6 | - |
4-coumarate | pH 7.5, 23°C | Escherichia coli | |
1.5 | - |
2-(4-hydroxyphenyl)ethanol | pH 7.5, 23°C | Escherichia coli | |
2.1 | - |
(3,4-dihydroxyphenyl)acetate | pH 7.5, 23°C | Escherichia coli | |
2.7 | - |
DL-4-hydroxymandelic acid | pH 7.5, 23°C | Escherichia coli | |
4.4 | - |
3-hydroxyphenylacetate | pH 7.5, 23°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
methyl 4-hydroxybenzoate | pH 7.5, 23°C | Escherichia coli | |
1.1 | - |
phenol | pH 7.5, 23°C | Escherichia coli | |
11 | - |
4-coumarate | pH 7.5, 23°C | Escherichia coli | |
44 | - |
2-(4-hydroxyphenyl)ethanol | pH 7.5, 23°C | Escherichia coli | |
46 | - |
(3,4-dihydroxyphenyl)acetate | pH 7.5, 23°C | Escherichia coli | |
110 | - |
DL-4-hydroxymandelic acid | pH 7.5, 23°C | Escherichia coli | |
470 | - |
3-hydroxyphenylacetate | pH 7.5, 23°C | Escherichia coli |