Cloned (Comment) | Organism |
---|---|
hpaB gene is expressed in Escherichia coli BL21(DE3) | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strain W, ATCC 11105 derived from ATCC 9637 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, ion exchange | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O | new type of FADH-utilizing monooxygenase | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.231 | - |
for 4-hydroxyphenylacetate oxidation | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NADH + O2 | O2 can be replaced by FADH | Escherichia coli | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
additional information | hydroxylation of aromatic compounds | Escherichia coli | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | reduction of FAD is rate limiting | Escherichia coli | |
FADH2 | used as substrate and cofactor, enzyme binds FADH in absence of 4-hydroxyphenlyacetate and protects it from rapid autoxidation by O2 | Escherichia coli |