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Literature summary for 1.14.14.126 extracted from
- The involvement of beta-amyrin 28-oxidase (Cyp716a52v2) in oleanane-type ginsenoside biosynthesis in Panax ginseng (2013), Plant Cell Physiol., 54, 2034-2046.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| coexpression of PNY1 and CYP716A52v2 in yeast. Transgenic Panax ginseng plants constitutively overexpressing CYP716A52v2 and expressing a CYP716A52v2-RNAi are generated using Agrobacterium tumefaciens mediated genetic transformation | Panax ginseng |
| expression in Saccharomyces cerevisiae | Panax ginseng |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, calculated | Panax ginseng |
| 54000 | - |
x * 54000, calculated from sequence | Panax ginseng |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | Panax ginseng | the enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro | oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Panax ginseng | I7C6E8 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | very low levels of mRNA in other organs than rhizome | Panax ginseng | - |
| rhizome | - |
Panax ginseng | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Panax ginseng | oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro | Panax ginseng | oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, calculated | Panax ginseng |
| ? | x * 54000, calculated from sequence | Panax ginseng |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-amyrin 28-oxidase | - |
Panax ginseng |
| CYP716A52v2 | - |
Panax ginseng |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Panax ginseng |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Panax ginseng |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Panax ginseng | CYP716A52v2 mRNA does not respond to methyl jasmonate treatment during the entire culture period | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro | Panax ginseng |
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