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Literature summary for 1.14.14.111 extracted from

  • Wang, Q.; Hillwig, M.; Peters, R.
    CYP99A3: Functional identification of a diterpene oxidase from the momilactone biosynthetic gene cluster in rice (2011), Plant J., 65, 87-95.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.111

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41 cells Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 syn-pimara-7,15-diene pH and temperature not specified in the publication Oryza sativa
0.009
-
 syn-stemod-13(17)-ene pH and temperature not specified in the publication Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q0JF01 cultivar Nipponbare
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CYP99A3 catalyzes consecutive oxidations of the C19 methyl group of the momilactone precursor syn-pimara-7,15-diene to form, sequentially, syn-pimaradien-19-ol, syn-pimaradien-19-al, and syn-pimaradien-19-oate Oryza sativa ?
-
 ?
syn-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa syn-pimaradien-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
syn-pimaradien-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa syn-pimaradien-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
syn-pimaradien-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa syn-pimaradien-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
syn-stemod-13(17)-ene + [reduced NADPH-hemoprotein reductase] + O2 about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19 Oryza sativa syn-stemoden-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
syn-stemoden-19-al + [reduced NADPH-hemoprotein reductase] + O2 about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19 Oryza sativa syn-stemoden-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
syn-stemoden-19-ol + [reduced NADPH-hemoprotein reductase] + O2 about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19 Oryza sativa syn-stemoden-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP99A3
-
 Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
46
-
 syn-pimara-7,15-diene pH and temperature not specified in the publication Oryza sativa
49
-
 syn-stemod-13(17)-ene pH and temperature not specified in the publication Oryza sativa

General Information

General Information Comment Organism
physiological function CYP99A3 acts as a multifunctional diterpene oxidase in momilactone biosynthesis Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5000
-
 syn-stemod-13(17)-ene pH and temperature not specified in the publication Oryza sativa
23000
-
 syn-pimara-7,15-diene pH and temperature not specified in the publication Oryza sativa