Application | Comment | Organism |
---|---|---|
synthesis | the thermally stable phenylacetone monooxygenase and engineered mutants can be used as a practical catalysts for enantioselective Baeyer-Villiger oxidations of several ketones on a preparative scale under in vitro conditions, overview | Thermobifida fusca |
Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering of three highly stereoselective mutants of the thermally stable phenylacetone monooxygenase as practical catalysts for enantioselective Baeyer-Villiger oxidations of several ketones on a preparative scale under in vitro conditions, optimization of the method including a coupled cofactor-regeneration system, reaction mechanism, overview | Thermobifida fusca |
General Stability | Organism |
---|---|
detergents stabilize the enzyme, best by Tween-20 at 0.1% v/v | Thermobifida fusca |
Organic Solvent | Comment | Organism |
---|---|---|
2-pentanol | as a sacrificial substrate | Thermobifida fusca |
cyclohexane | stabilizes the enzyme, best organic solvent, inactivation of the enzyme within 5 min | Thermobifida fusca |
isopropanol | the most effective stoichiometric sacrificial electron donor, optimal at 5% v/v | Thermobifida fusca |
methyl tert-butyl ether | stabilizes the enzyme, inactivation of the enzyme within below 5 min | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
catalytic efficiency of wild-type and mutant PAMO in in vitro catalysis with two-liquid phases, overview | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
rac-bicyclo [3.2.0]hept-2-en-6-one + NADPH + O2 | activity and stereoselectivity of wild-type and mutant enzymes, overview | Thermobifida fusca | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAMO | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Thermobifida fusca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Thermobifida fusca |