Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Pseudomonas putida | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 30% stimulation at 4 mM | Pseudomonas putida | |
Mg2+ | 50% stimulation at 4 mM, EDTA reverses stimulation | Pseudomonas putida | |
Mn2+ | 50% stimulation at 4 mM | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenol + NADPH + O2 | Pseudomonas putida | involved in metabolism of nitro-aromatic compounds by a strain of Pseudomonas putida | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida B2 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
with 4-chloro-2-nitrophenol as substrate | Pseudomonas putida |
50 | - |
with 4-methyl-2-nitrophenol as substrate | Pseudomonas putida |
60 | - |
with 2-nitrophenol as substrate | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenol + NADPH + H+ + O2 | - |
Pseudomonas putida | catechol + nitrite + NADP+ + H2O | - |
? | |
2-nitrophenol + NADPH + H+ + O2 | - |
Pseudomonas putida B2 | catechol + nitrite + NADP+ + H2O | - |
? | |
2-nitrophenol + NADPH + O2 | involved in metabolism of nitro-aromatic compounds by a strain of Pseudomonas putida | Pseudomonas putida | ? | - |
? | |
4-chloro-2-nitrophenol + NADPH + O2 | - |
Pseudomonas putida | 4-chlorocatechol + NADP+ + H2O | - |
? | |
4-chloro-2-nitrophenol + NADPH + O2 | - |
Pseudomonas putida B2 | 4-chlorocatechol + NADP+ + H2O | - |
? | |
4-formyl-2-nitrophenol + NADPH + O2 | weak activity | Pseudomonas putida | 4-formyl-1,2-benzenediol + NADP+ + H2O | - |
? | |
4-formyl-2-nitrophenol + NADPH + O2 | weak activity | Pseudomonas putida B2 | 4-formyl-1,2-benzenediol + NADP+ + H2O | - |
? | |
4-methyl-2-nitrophenol + NADPH + O2 | - |
Pseudomonas putida | 4-methylcatechol + NADP+ + H2O | - |
? | |
4-methyl-2-nitrophenol + NADPH + O2 | - |
Pseudomonas putida B2 | 4-methylcatechol + NADP+ + H2O | - |
? | |
additional information | not: 4-carboxy-2-nitrophenol, 4-nitro-2-nitrophenol | Pseudomonas putida | ? | - |
? | |
additional information | not: 4-carboxy-2-nitrophenol, 4-nitro-2-nitrophenol | Pseudomonas putida B2 | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Pseudomonas putida |