Literature summary for 1.14.13.230 extracted from

  • Doughty, D.M.; Halsey, K.H.; Vieville, C.J.; Sayavedra-Soto, L.A.; Arp, D.J.; Bottomley, P.J.
    Propionate inactivation of butane monooxygenase activity in Pseudomonas butanovora: biochemical and physiological implications (2007), Microbiology, 153, 3722-3729.
    View publication on PubMed

Engineering

Protein Variants Comment Organism
G113N mutation in the alpha-subunit of hydrolase component, loss of inhibition by propanoate Thauera butanivorans
T148C mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 15% residual activity Thauera butanivorans
Q320K mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 28% residual activity Thauera butanivorans
F321Y mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 61% residual activity Thauera butanivorans
L279F mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 56% residual activity Thauera butanivorans

Inhibitors

Inhibitors Comment Organism Structure
Butanoate incubation of alkane-grown cells with butanoate or propanoate leads to irreversible time- and O2-dependent loss of butane monooxygenase activity Thauera butanivorans
propanoate 10 mM, 41% residual activity. Incubation of alkane-grown cells with butanoate or propanoate leads to irreversible time- and O2-dependent loss of butane monooxygenase activity. Propanoate-dependent inactivation involves interaction with the catalytic site. Butane monooxygenase is protected from propanoate-dependent inactivation by the presence of its natural substrate, butane Thauera butanivorans

Organism

Organism UniProt Comment Textmining
Thauera butanivorans Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8 Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively. Q8KQE6 i.e. oxidoreductase BmoC, Q8KQE8 i.e. regulatory protein BmoB
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Thauera butanivorans ATCC 43655 Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8 Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively. Q8KQE6 i.e. oxidoreductase BmoC, Q8KQE8 i.e. regulatory protein BmoB
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