Literature summary for 1.14.13.230 extracted from

  • Halsey, K.H.; Sayavedra-Soto, L.A.; Bottomley, P.J.; Arp, D.J.
    Site-directed amino acid substitutions in the hydroxylase alpha subunit of butane monooxygenase from Pseudomonas butanovora: Implications for substrates knocking at the gate (2006), J. Bacteriol., 188, 4962-4969.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
V181M mutation in subunit BmoX, loss of activity Thauera butanivorans
G113N mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 11.1 Thauera butanivorans
Q319G mutation in subunit BmoX, loss of activity Thauera butanivorans
P179K mutation in subunit BmoX, loss of activity Thauera butanivorans
L287F mutation in subunit BmoX, loss of activity Thauera butanivorans
T148C mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.15 Thauera butanivorans
Q320K mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.20 Thauera butanivorans
F321Y mutation in subunit BmoX towards soluble methane monooxygenase, no formation of butan-2-ol Thauera butanivorans
L279F mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.22 Thauera butanivorans

Organism

Organism UniProt Comment Textmining
Thauera butanivorans Q8KQF0 and Q8KQE9 and Q8KQE7 Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butane + NADH + H+ + O2
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Thauera butanivorans butan-1-ol + NAD+ + H2O formation of about 3.6% of butan-2-ol ?

Synonyms

Synonyms Comment Organism
BmoX
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Thauera butanivorans
BmoY
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Thauera butanivorans
BmoZ
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Thauera butanivorans

General Information

General Information Comment Organism
physiological function except for C1, strain BCP1 cells grow on the tested short-chain n-alkanes and show the lowest generation times with C3-C5 n-alkanes (2.7รขย€ย“2.8 h) Thauera butanivorans