BRENDA - Enzyme Database show
show all sequences of 1.14.13.20

Purification and properties of a plasmid-encoded 2,4-dichlorophenol hydroxylase

Liu, T.; Chapman, P.J.; FEBS Lett. 173, 314-318 (1984)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Pseudomonas putida
Cupriavidus necator
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,4-Dichlorophenol
above 1 mM
Cupriavidus necator
AgNO3
-
Cupriavidus necator
CuSO4
-
Cupriavidus necator
diethyldithiocarbamate
-
Cupriavidus necator
Dithionitrobenzoate
-
Cupriavidus necator
dithiothreitol
slight
Cupriavidus necator
FeSO4
-
Cupriavidus necator
Hg2+
HgCl2
Cupriavidus necator
KCl
high salt concentration inhibits, 0.05 M: 40%, 0.1 M: 50%, 0.2 M: 70%
Cupriavidus necator
KCN
no effect
Cupriavidus necator
o-phenanthroline
-
Cupriavidus necator
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
2,4-Dichlorophenol
cosubstrate NADPH
Cupriavidus necator
0.004
-
2,4-Dichlorophenol
cosubstrate NADH
Cupriavidus necator
0.048
-
NADPH
+ 2,4-dichlorophenol
Cupriavidus necator
0.3
-
NADH
+ 2,4-dichlorophenol
Cupriavidus necator
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
alpha2beta2, 2 * 67000 + 2 * 45000, SDS-PAGE
Cupriavidus necator
67000
-
alpha2beta2, 2 * 67000 + 2 * 45000, SDS-PAGE
Cupriavidus necator
224000
-
ultracentrifugation
Cupriavidus necator
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,4-dichlorophenol + NADPH + O2
Cupriavidus necator
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
2,4-dichlorophenol + NADPH + O2
Cupriavidus necator JMP 134-1
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cupriavidus necator
-
basonym Alcaligenes eutrophus; JMP134
-
Cupriavidus necator JMP 134-1
-
JMP134
-
Purification (Commentary)
Commentary
Organism
plasmid pJP4-encoded enzyme from Alcaligenes eutrophus JMP134 transferred to Pseudomonas putida 3CB5 and Alcaligenes eutrophus 335
Cupriavidus necator
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.48
-
-
Cupriavidus necator
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4,5-trichlorophenol + NADPH + O2
-
439012
Cupriavidus necator
?
-
-
-
?
2,4,5-trichlorophenol + NADPH + O2
-
439012
Cupriavidus necator JMP 134-1
?
-
-
-
?
2,4-dibromophenol + NADPH + O2
-
439012
Cupriavidus necator
?
-
-
-
?
2,4-dibromophenol + NADPH + O2
-
439012
Cupriavidus necator JMP 134-1
?
-
-
-
?
2,4-dichlorophenol + NADPH + O2
-
439012
Cupriavidus necator
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
-
439012
Cupriavidus necator JMP 134-1
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2-chloro-4-nitrophenol + NADPH + O2
-
439012
Cupriavidus necator
?
-
-
-
?
2-chlorophenol + NADPH + O2
incompletely metabolized
439012
Cupriavidus necator
?
-
-
-
?
2-chlorophenol + NADPH + O2
incompletely metabolized
439012
Cupriavidus necator JMP 134-1
?
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
-
439012
Cupriavidus necator
?
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
-
439012
Cupriavidus necator JMP 134-1
?
-
-
-
?
4-chloro-2-nitrophenol + NADPH + O2
-
439012
Cupriavidus necator
?
-
-
-
?
4-chlorophenol + NADPH + O2
-
439012
Cupriavidus necator
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
alpha2beta2, 2 * 67000 + 2 * 45000, SDS-PAGE
Cupriavidus necator
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
8
phosphate buffer
Cupriavidus necator
8.1
8.5
100 mM Tris-HCl buffer
Cupriavidus necator
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
1 mol of enzyme contains: 3.5 mol FAD; flavoprotein
Cupriavidus necator
NADH
50% of the activity with NADPH
Cupriavidus necator
NADPH
-
Cupriavidus necator
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Pseudomonas putida
Cupriavidus necator
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
1 mol of enzyme contains: 3.5 mol FAD; flavoprotein
Cupriavidus necator
NADH
50% of the activity with NADPH
Cupriavidus necator
NADPH
-
Cupriavidus necator
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,4-Dichlorophenol
above 1 mM
Cupriavidus necator
AgNO3
-
Cupriavidus necator
CuSO4
-
Cupriavidus necator
diethyldithiocarbamate
-
Cupriavidus necator
Dithionitrobenzoate
-
Cupriavidus necator
dithiothreitol
slight
Cupriavidus necator
FeSO4
-
Cupriavidus necator
Hg2+
HgCl2
Cupriavidus necator
KCl
high salt concentration inhibits, 0.05 M: 40%, 0.1 M: 50%, 0.2 M: 70%
Cupriavidus necator
KCN
no effect
Cupriavidus necator
o-phenanthroline
-
Cupriavidus necator
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
2,4-Dichlorophenol
cosubstrate NADPH
Cupriavidus necator
0.004
-
2,4-Dichlorophenol
cosubstrate NADH
Cupriavidus necator
0.048
-
NADPH
+ 2,4-dichlorophenol
Cupriavidus necator
0.3
-
NADH
+ 2,4-dichlorophenol
Cupriavidus necator
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
alpha2beta2, 2 * 67000 + 2 * 45000, SDS-PAGE
Cupriavidus necator
67000
-
alpha2beta2, 2 * 67000 + 2 * 45000, SDS-PAGE
Cupriavidus necator
224000
-
ultracentrifugation
Cupriavidus necator
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,4-dichlorophenol + NADPH + O2
Cupriavidus necator
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
2,4-dichlorophenol + NADPH + O2
Cupriavidus necator JMP 134-1
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
plasmid pJP4-encoded enzyme from Alcaligenes eutrophus JMP134 transferred to Pseudomonas putida 3CB5 and Alcaligenes eutrophus 335
Cupriavidus necator
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.48
-
-
Cupriavidus necator
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4,5-trichlorophenol + NADPH + O2
-
439012
Cupriavidus necator
?
-
-
-
?
2,4,5-trichlorophenol + NADPH + O2
-
439012
Cupriavidus necator JMP 134-1
?
-
-
-
?
2,4-dibromophenol + NADPH + O2
-
439012
Cupriavidus necator
?
-
-
-
?
2,4-dibromophenol + NADPH + O2
-
439012
Cupriavidus necator JMP 134-1
?
-
-
-
?
2,4-dichlorophenol + NADPH + O2
-
439012
Cupriavidus necator
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
-
439012
Cupriavidus necator JMP 134-1
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2-chloro-4-nitrophenol + NADPH + O2
-
439012
Cupriavidus necator
?
-
-
-
?
2-chlorophenol + NADPH + O2
incompletely metabolized
439012
Cupriavidus necator
?
-
-
-
?
2-chlorophenol + NADPH + O2
incompletely metabolized
439012
Cupriavidus necator JMP 134-1
?
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
-
439012
Cupriavidus necator
?
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
-
439012
Cupriavidus necator JMP 134-1
?
-
-
-
?
4-chloro-2-nitrophenol + NADPH + O2
-
439012
Cupriavidus necator
?
-
-
-
?
4-chlorophenol + NADPH + O2
-
439012
Cupriavidus necator
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
alpha2beta2, 2 * 67000 + 2 * 45000, SDS-PAGE
Cupriavidus necator
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
8
phosphate buffer
Cupriavidus necator
8.1
8.5
100 mM Tris-HCl buffer
Cupriavidus necator
Other publictions for EC 1.14.13.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742330
Xia
Biodegradation of the Herbici ...
Achromobacter sp. LZ35
Curr. Microbiol.
74
193-202
2017
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1
1
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724636
Lu
Cloning and characterisation o ...
Soil bacterium
Biotechnol. Lett.
33
1159-1167
2011
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-
1
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3
2
-
3
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3
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9
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1
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1
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1
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3
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2
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3
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9
-
1
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-
1
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-
685661
Huong
Chlorophenol hydroxylase activ ...
Bradyrhizobium sp., Bradyrhizobium sp. RD5-C2
Biosci. Biotechnol. Biochem.
71
1691-1696
2007
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
10
-
1
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1
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-
1
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1
1
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-
-
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-
2
-
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-
-
10
-
1
-
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-
1
-
-
-
-
-
-
-
-
-
671456
Ledger
Chlorophenol hydroxylases enco ...
Cupriavidus necator, Paraburkholderia xenovorans
Appl. Environ. Microbiol.
72
2783-2792
2006
-
-
-
-
2
-
-
1
-
-
-
8
-
7
-
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1
-
8
-
2
-
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-
2
-
-
2
-
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2
-
2
-
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1
-
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-
8
-
-
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-
-
-
1
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
439014
Farhana
Monoclonal antibodies to 2,4-d ...
Burkholderia cepacia, Burkholderia cepacia DBO131, Burkholderia mallei, Burkholderia mallei TFD6, Burkholderia sp., Burkholderia sp. RASC, Burkholderia sp. TFD2, Cupriavidus necator, Cupriavidus necator JMP 134-1, Cupriavidus necator TFD41, Pseudomonas putida, Pseudomonas putida PaW85, Rhodoferax fermentans, Rhodoferax fermentans B6-9, Rhodoferax fermentans TFD23, Rhodopseudomonas palustris, Rhodopseudomonas palustris M1, Sphingomonas paucimobilis 1443, Sphingomonas paucimobilis, Sphingomonas sp., Sphingomonas sp. B6-10, Sphingomonas sp. B6-5, Sphingomonas sp. EML146, Sphingomonas sp. TFD44, Variovorax paradoxus 2811P, Variovorax paradoxus
Can. J. Microbiol.
44
920-928
1998
-
-
-
-
-
-
-
-
-
-
-
26
-
54
-
-
1
-
-
-
-
-
26
-
-
-
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-
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-
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26
-
-
-
1
-
-
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-
26
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439015
Farhana
The 2,4-dichlorophenol hydroxy ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
Can. J. Microbiol.
43
202-205
1997
-
-
-
-
-
-
-
-
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-
2
2
-
28
-
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1
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1
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2
1
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2
2
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1
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1
-
2
1
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-
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-
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-
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439016
Makdessi
Purification and characterizat ...
Proteobacteria, Proteobacteria S1
FEMS Microbiol. Lett.
157
95-101
1997
-
-
-
-
-
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-
3
-
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2
2
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5
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1
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2
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20
1
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3
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3
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3
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2
2
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1
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2
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20
1
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439013
Radjendirane
Affinity purification and char ...
Burkholderia cepacia
Arch. Biochem. Biophys.
288
169-176
1991
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8
2
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2
1
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3
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1
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1
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1
1
1
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1
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3
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3
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8
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2
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2
1
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1
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1
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1
1
1
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1
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-
439012
Liu
Purification and properties of ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
FEBS Lett.
173
314-318
1984
-
-
1
-
-
-
11
4
-
-
3
2
-
27
-
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1
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1
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13
1
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2
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3
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1
3
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11
-
4
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3
2
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1
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1
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13
1
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2
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439011
Beadle
The purification and propertie ...
Acinetobacter sp.
Eur. J. Biochem.
123
323-332
1982
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4
10
3
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2
1
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3
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1
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1
1
7
1
1
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1
1
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3
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3
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4
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10
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3
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2
1
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1
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1
1
7
1
1
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1
1
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