Cloned (Comment) | Organism |
---|---|
expression in Pseudomonas putida | Cupriavidus necator |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-Dichlorophenol | above 1 mM | Cupriavidus necator | |
AgNO3 | - |
Cupriavidus necator | |
CuSO4 | - |
Cupriavidus necator | |
diethyldithiocarbamate | - |
Cupriavidus necator | |
Dithionitrobenzoate | - |
Cupriavidus necator | |
dithiothreitol | slight | Cupriavidus necator | |
FeSO4 | - |
Cupriavidus necator | |
Hg2+ | HgCl2 | Cupriavidus necator | |
KCl | high salt concentration inhibits, 0.05 M: 40%, 0.1 M: 50%, 0.2 M: 70% | Cupriavidus necator | |
KCN | no effect | Cupriavidus necator | |
o-phenanthroline | - |
Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
2,4-Dichlorophenol | cosubstrate NADPH | Cupriavidus necator | |
0.004 | - |
2,4-Dichlorophenol | cosubstrate NADH | Cupriavidus necator | |
0.048 | - |
NADPH | + 2,4-dichlorophenol | Cupriavidus necator | |
0.3 | - |
NADH | + 2,4-dichlorophenol | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
alpha2beta2, 2 * 67000 + 2 * 45000, SDS-PAGE | Cupriavidus necator |
67000 | - |
alpha2beta2, 2 * 67000 + 2 * 45000, SDS-PAGE | Cupriavidus necator |
224000 | - |
ultracentrifugation | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichlorophenol + NADPH + O2 | Cupriavidus necator | - |
3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
2,4-dichlorophenol + NADPH + O2 | Cupriavidus necator JMP 134-1 | - |
3,5-dichlorocatechol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
basonym Alcaligenes eutrophus | - |
Cupriavidus necator | - |
JMP134 | - |
Cupriavidus necator JMP 134-1 | - |
JMP134 | - |
Purification (Comment) | Organism |
---|---|
plasmid pJP4-encoded enzyme from Alcaligenes eutrophus JMP134 transferred to Pseudomonas putida 3CB5 and Alcaligenes eutrophus 335 | Cupriavidus necator |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.48 | - |
- |
Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4,5-trichlorophenol + NADPH + O2 | - |
Cupriavidus necator | ? | - |
? | |
2,4,5-trichlorophenol + NADPH + O2 | - |
Cupriavidus necator JMP 134-1 | ? | - |
? | |
2,4-dibromophenol + NADPH + O2 | - |
Cupriavidus necator | ? | - |
? | |
2,4-dibromophenol + NADPH + O2 | - |
Cupriavidus necator JMP 134-1 | ? | - |
? | |
2,4-dichlorophenol + NADH + H+ + O2 | - |
Cupriavidus necator | 3,5-dichlorocatechol + NAD+ + H2O | - |
r | |
2,4-dichlorophenol + NADPH + O2 | - |
Cupriavidus necator | 3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
2,4-dichlorophenol + NADPH + O2 | - |
Cupriavidus necator JMP 134-1 | 3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
2-chloro-4-nitrophenol + NADPH + O2 | - |
Cupriavidus necator | ? | - |
? | |
2-chlorophenol + NADPH + O2 | incompletely metabolized | Cupriavidus necator | ? | - |
? | |
2-chlorophenol + NADPH + O2 | incompletely metabolized | Cupriavidus necator JMP 134-1 | ? | - |
? | |
4-chloro-2-methylphenol + NADPH + O2 | - |
Cupriavidus necator | ? | - |
? | |
4-chloro-2-methylphenol + NADPH + O2 | - |
Cupriavidus necator JMP 134-1 | ? | - |
? | |
4-chloro-2-nitrophenol + NADPH + O2 | - |
Cupriavidus necator | ? | - |
? | |
4-chlorophenol + NADPH + O2 | - |
Cupriavidus necator | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha2beta2, 2 * 67000 + 2 * 45000, SDS-PAGE | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 8 | phosphate buffer | Cupriavidus necator |
8.1 | 8.5 | 100 mM Tris-HCl buffer | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Cupriavidus necator | |
FAD | 1 mol of enzyme contains: 3.5 mol FAD | Cupriavidus necator | |
NADH | 50% of the activity with NADPH | Cupriavidus necator | |
NADPH | - |
Cupriavidus necator |